Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival

C. Rastellini, L. Lu, C. Ricorbi, T. E. Starzl, A. S. Rao, A. W. Thomson

Research output: Contribution to journalArticle

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Abstract

Liver-derived dendritic cell (DC) progenitors propagated in liquid culture in granulocyte/macrophage colony-stimulating factor exhibit low levels both of cell surface MHC class II antigens and of counter-receptors for CTLA- 4/CD28. They fail to stimulate allogeneic T cells in mixed leukocyte cultures. To evaluate their in vivo functional significance, we determined their influence on survival of pancreatic islet allografts. Cultured B10.BR (H2(k); I-E+) mouse liver-derived DC progenitors were injected (2x106 i.v.) into streptozotocin-diabetic B10 (H2(b); I-E) recipients 7 days before transplantation of pancreatic islets (700 IEq/mouse) from the same donor strain. No immunosuppressive agents were administered. Mean islet allograft survival time was prolonged from 15.3 days (in animals pretreated with syngeneic cells) to 30.3 days (P<0.001) in mice pretreated with the donor- derived liver cells. In 20% of these animals, islet allograft survival exceeded 60 days. These data suggest that liver-derived DC progenitors may contribute both to the inherent tolerogenicity of the mouse liver and to its capacity to protect other allografts of the same donor strain from rejection.

Original languageEnglish (US)
Pages (from-to)1366-1370
Number of pages5
JournalTransplantation
Volume60
Issue number11
StatePublished - Dec 1 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Transplantation

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