Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro

Xin-Hai Pei, Yoichi Nakanishi, Koichi Takayama, Jun Yatsunami, Feng Bai, Masayuki Kawasaki, Kentaro Wakamatsu, Nobuko Tsuruta, Keiko Mizuno, Nobuyuki Hara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of exogenous and endogenous granulocyte colony-stimulating factor (G-CSF) on invasion by cancer cells were studied, using lung cancer cell lines that produce G-CSF (NCI-H157) and lines that do not (PC-9 and NCI-H23). The invasive capacity of NCI-H157 cells was 26- to 27-fold higher than that of PC-9 and NCI-H23 cells. The invasiveness of PC-9 cells was stimulated by exogenous G-CSF, while that of NCI-H157 cells was not. Antibodies against G-CSF blocked the stimulation of PC-9 cell invasiveness by exogenous G-CSF. Anti G-CSF antibodies also inhibited invasion by NCI-H157 cells in the absence of exogenous G-CSF. These results suggest that endogenous and exogenous G-CSF both stimulate invasion by lung cancer cells.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalClinical and Experimental Metastasis
Volume14
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

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Granulocyte Colony-Stimulating Factor
Lung Neoplasms
Cell Line
In Vitro Techniques
Antibodies

Keywords

  • Granulocyte-colony stimulating factor
  • Invasion
  • Invasion assay
  • Lung cancer

ASJC Scopus subject areas

  • Cancer Research

Cite this

Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro. / Pei, Xin-Hai; Nakanishi, Yoichi; Takayama, Koichi; Yatsunami, Jun; Bai, Feng; Kawasaki, Masayuki; Wakamatsu, Kentaro; Tsuruta, Nobuko; Mizuno, Keiko; Hara, Nobuyuki.

In: Clinical and Experimental Metastasis, Vol. 14, No. 4, 1996, p. 351-357.

Research output: Contribution to journalArticle

Pei, X-H, Nakanishi, Y, Takayama, K, Yatsunami, J, Bai, F, Kawasaki, M, Wakamatsu, K, Tsuruta, N, Mizuno, K & Hara, N 1996, 'Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro', Clinical and Experimental Metastasis, vol. 14, no. 4, pp. 351-357. https://doi.org/10.1007/BF00123394
Pei, Xin-Hai ; Nakanishi, Yoichi ; Takayama, Koichi ; Yatsunami, Jun ; Bai, Feng ; Kawasaki, Masayuki ; Wakamatsu, Kentaro ; Tsuruta, Nobuko ; Mizuno, Keiko ; Hara, Nobuyuki. / Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro. In: Clinical and Experimental Metastasis. 1996 ; Vol. 14, No. 4. pp. 351-357.
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AU - Bai, Feng

AU - Kawasaki, Masayuki

AU - Wakamatsu, Kentaro

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AU - Mizuno, Keiko

AU - Hara, Nobuyuki

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