Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro

Xin Hai Pei, Yoichi Nakanishi, Koichi Takayama, Jun Yatsunami, Feng Bai, Masayuki Kawasaki, Kentaro Wakamatsu, Nobuko Tsuruta, Keiko Mizuno, Nobuyuki Hara

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The effects of exogenous and endogenous granulocyte colony-stimulating factor (G-CSF) on invasion by cancer cells were studied, using lung cancer cell lines that produce G-CSF (NCI-H157) and lines that do not (PC-9 and NCI-H23). The invasive capacity of NCI-H157 cells was 26- to 27-fold higher than that of PC-9 and NCI-H23 cells. The invasiveness of PC-9 cells was stimulated by exogenous G-CSF, while that of NCI-H157 cells was not. Antibodies against G-CSF blocked the stimulation of PC-9 cell invasiveness by exogenous G-CSF. Anti G-CSF antibodies also inhibited invasion by NCI-H157 cells in the absence of exogenous G-CSF. These results suggest that endogenous and exogenous G-CSF both stimulate invasion by lung cancer cells.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalClinical and Experimental Metastasis
Issue number4
StatePublished - 1996
Externally publishedYes


  • Granulocyte-colony stimulating factor
  • Invasion
  • Invasion assay
  • Lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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