Graded contusion model of the mouse spinal cord using a pneumatic impact device

Toshitaka Seki, Kazutoshi Hida, Mitsuhiro Tada, Izumi Koyanagi, Yoshinobu Iwasaki, Michael Y. Wang, Alexander Marcillo, Edward C. Benzel

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

OBJECTIVE: This study examined the effects of varying magnitudes of controlled spinal cord impact to the mouse spinal cord on neurological and histopathological variables to obtain a mouse model of spinal cord injury (SCI). METHODS: A laminectomy of the T10 vertebra was performed on anesthetized C57BL/6 mice. A pneumatic pressure-driven impact was performed on the spinal cord through the dura mater. Experimental groups were subdivided according to the energy of impact (0.25-mm-deep deformations): Group 1 (n = 5), impact velocity at 1 m/s; Group 2 (n = 5), impact velocity at 2 m/s; and Group 3 (n = 5), impact velocity at 3 m/s. Functional deficits over time were evaluated up to 28 days after SCI by testing hindlimb reflex and coordinated motor function. The extent of the lesions was analyzed histopathologically and quantified by a morphometric measurement. RESULTS: Mice of all groups exhibited profound functional deficits immediately after injury and subsequent gradual symptomatic recovery. The degrees of recovery were precisely correlated with the magnitudes of impact. The extent of resultant cord lesions was highly reproducible among animals, with little variance: means ± standard deviation, 0.86 ± 0.06/100 mm3 in Group 1; 2.4 ± 0.28/100 mm3 in Group 2; and 11.0 ± 1.0/100 mm3 in Group 3. CONCLUSION: Our results indicate that this model provides constant functional and histopathological lesions according to impact energy. This new mouse model of SCI opens a new avenue for studies investigating roles and/or effects of specific genes in the recovery process of SCI.

Original languageEnglish
Pages (from-to)1075-1082
Number of pages8
JournalNeurosurgery
Volume50
Issue number5
DOIs
StatePublished - May 1 2002
Externally publishedYes

Fingerprint

Contusions
Spinal Cord Injuries
Spinal Cord
Equipment and Supplies
Dura Mater
Laminectomy
Hindlimb
Inbred C57BL Mouse
Reflex
Spine
Pressure
Wounds and Injuries
Genes

Keywords

  • Mouse model
  • Pneumatic impact device
  • Spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Seki, T., Hida, K., Tada, M., Koyanagi, I., Iwasaki, Y., Wang, M. Y., ... Benzel, E. C. (2002). Graded contusion model of the mouse spinal cord using a pneumatic impact device. Neurosurgery, 50(5), 1075-1082. https://doi.org/10.1097/00006123-200205000-00024

Graded contusion model of the mouse spinal cord using a pneumatic impact device. / Seki, Toshitaka; Hida, Kazutoshi; Tada, Mitsuhiro; Koyanagi, Izumi; Iwasaki, Yoshinobu; Wang, Michael Y.; Marcillo, Alexander; Benzel, Edward C.

In: Neurosurgery, Vol. 50, No. 5, 01.05.2002, p. 1075-1082.

Research output: Contribution to journalArticle

Seki, T, Hida, K, Tada, M, Koyanagi, I, Iwasaki, Y, Wang, MY, Marcillo, A & Benzel, EC 2002, 'Graded contusion model of the mouse spinal cord using a pneumatic impact device', Neurosurgery, vol. 50, no. 5, pp. 1075-1082. https://doi.org/10.1097/00006123-200205000-00024
Seki T, Hida K, Tada M, Koyanagi I, Iwasaki Y, Wang MY et al. Graded contusion model of the mouse spinal cord using a pneumatic impact device. Neurosurgery. 2002 May 1;50(5):1075-1082. https://doi.org/10.1097/00006123-200205000-00024
Seki, Toshitaka ; Hida, Kazutoshi ; Tada, Mitsuhiro ; Koyanagi, Izumi ; Iwasaki, Yoshinobu ; Wang, Michael Y. ; Marcillo, Alexander ; Benzel, Edward C. / Graded contusion model of the mouse spinal cord using a pneumatic impact device. In: Neurosurgery. 2002 ; Vol. 50, No. 5. pp. 1075-1082.
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AB - OBJECTIVE: This study examined the effects of varying magnitudes of controlled spinal cord impact to the mouse spinal cord on neurological and histopathological variables to obtain a mouse model of spinal cord injury (SCI). METHODS: A laminectomy of the T10 vertebra was performed on anesthetized C57BL/6 mice. A pneumatic pressure-driven impact was performed on the spinal cord through the dura mater. Experimental groups were subdivided according to the energy of impact (0.25-mm-deep deformations): Group 1 (n = 5), impact velocity at 1 m/s; Group 2 (n = 5), impact velocity at 2 m/s; and Group 3 (n = 5), impact velocity at 3 m/s. Functional deficits over time were evaluated up to 28 days after SCI by testing hindlimb reflex and coordinated motor function. The extent of the lesions was analyzed histopathologically and quantified by a morphometric measurement. RESULTS: Mice of all groups exhibited profound functional deficits immediately after injury and subsequent gradual symptomatic recovery. The degrees of recovery were precisely correlated with the magnitudes of impact. The extent of resultant cord lesions was highly reproducible among animals, with little variance: means ± standard deviation, 0.86 ± 0.06/100 mm3 in Group 1; 2.4 ± 0.28/100 mm3 in Group 2; and 11.0 ± 1.0/100 mm3 in Group 3. CONCLUSION: Our results indicate that this model provides constant functional and histopathological lesions according to impact energy. This new mouse model of SCI opens a new avenue for studies investigating roles and/or effects of specific genes in the recovery process of SCI.

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