GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice

Jennifer L. Owen, Marta Torroella-Kouri, Mary E. Handel-Fernandez, Vijaya Iragavarapu-Charyulu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

MCP-1/CCL2 (monocyte chemoattractant protein-1/CC chemokine ligand 2) is a β or CC chemokine that is expressed by a variety of cell types, including fibroblasts, endothelial, smooth muscle, and glial cells. In addition, cells involved in immunity, such as monocytes/macrophages, neutrophils, and eosinophils have also been shown to express this chemoattractant. Using a murine model of the D1-DMBA-3 mammary adenocarcinoma, we demonstrated the unique production of CCL2 by splenic T lymphocytes from tumor-bearing animals. Because this tumor produces GM-CSF, and this factor is also up-regulated in the B lymphocytes of tumor-bearing mice, we looked at the ability of GM-CSF to induce CCL2 production by T cells. Treatment of normal and tumor bearers' T cells with GM-CSF resulted in an increased secretion of this chemokine. This up-regulation was seen with or without stimulation by Concanavalin A, although these treatments were additive in their effects. The induction of CCL2 was studied at the molecular level by analyzing the effect(s) of a variety of physiological and pharmacological agents on cultured T cells. These results suggest that the tumor-derived factor GM-CSF activates various signaling pathways within splenic T cells to up-regulate CCL2 expression.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume20
Issue number1
StatePublished - Jul 1 2007

Fingerprint

Granulocyte-Macrophage Colony-Stimulating Factor
Up-Regulation
Breast Neoplasms
T-Lymphocytes
CC Chemokines
Neoplasms
9,10-Dimethyl-1,2-benzanthracene
Chemokine CCL2
Chemotactic Factors
Concanavalin A
Chemokines
Eosinophils
Neuroglia
Smooth Muscle Myocytes
Monocytes
Cultured Cells
Immunity
Adenocarcinoma
Breast
Neutrophils

Keywords

  • CCL2
  • GM-CSF
  • Mammary tumor
  • T lymphocytes

ASJC Scopus subject areas

  • Genetics

Cite this

Owen, J. L., Torroella-Kouri, M., Handel-Fernandez, M. E., & Iragavarapu-Charyulu, V. (2007). GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice. International Journal of Molecular Medicine, 20(1), 129-136.

GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice. / Owen, Jennifer L.; Torroella-Kouri, Marta; Handel-Fernandez, Mary E.; Iragavarapu-Charyulu, Vijaya.

In: International Journal of Molecular Medicine, Vol. 20, No. 1, 01.07.2007, p. 129-136.

Research output: Contribution to journalArticle

Owen, JL, Torroella-Kouri, M, Handel-Fernandez, ME & Iragavarapu-Charyulu, V 2007, 'GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice', International Journal of Molecular Medicine, vol. 20, no. 1, pp. 129-136.
Owen JL, Torroella-Kouri M, Handel-Fernandez ME, Iragavarapu-Charyulu V. GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice. International Journal of Molecular Medicine. 2007 Jul 1;20(1):129-136.
Owen, Jennifer L. ; Torroella-Kouri, Marta ; Handel-Fernandez, Mary E. ; Iragavarapu-Charyulu, Vijaya. / GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice. In: International Journal of Molecular Medicine. 2007 ; Vol. 20, No. 1. pp. 129-136.
@article{3170352ca71b475ba9285eeecf0b48a8,
title = "GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice",
abstract = "MCP-1/CCL2 (monocyte chemoattractant protein-1/CC chemokine ligand 2) is a β or CC chemokine that is expressed by a variety of cell types, including fibroblasts, endothelial, smooth muscle, and glial cells. In addition, cells involved in immunity, such as monocytes/macrophages, neutrophils, and eosinophils have also been shown to express this chemoattractant. Using a murine model of the D1-DMBA-3 mammary adenocarcinoma, we demonstrated the unique production of CCL2 by splenic T lymphocytes from tumor-bearing animals. Because this tumor produces GM-CSF, and this factor is also up-regulated in the B lymphocytes of tumor-bearing mice, we looked at the ability of GM-CSF to induce CCL2 production by T cells. Treatment of normal and tumor bearers' T cells with GM-CSF resulted in an increased secretion of this chemokine. This up-regulation was seen with or without stimulation by Concanavalin A, although these treatments were additive in their effects. The induction of CCL2 was studied at the molecular level by analyzing the effect(s) of a variety of physiological and pharmacological agents on cultured T cells. These results suggest that the tumor-derived factor GM-CSF activates various signaling pathways within splenic T cells to up-regulate CCL2 expression.",
keywords = "CCL2, GM-CSF, Mammary tumor, T lymphocytes",
author = "Owen, {Jennifer L.} and Marta Torroella-Kouri and Handel-Fernandez, {Mary E.} and Vijaya Iragavarapu-Charyulu",
year = "2007",
month = "7",
day = "1",
language = "English",
volume = "20",
pages = "129--136",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice

AU - Owen, Jennifer L.

AU - Torroella-Kouri, Marta

AU - Handel-Fernandez, Mary E.

AU - Iragavarapu-Charyulu, Vijaya

PY - 2007/7/1

Y1 - 2007/7/1

N2 - MCP-1/CCL2 (monocyte chemoattractant protein-1/CC chemokine ligand 2) is a β or CC chemokine that is expressed by a variety of cell types, including fibroblasts, endothelial, smooth muscle, and glial cells. In addition, cells involved in immunity, such as monocytes/macrophages, neutrophils, and eosinophils have also been shown to express this chemoattractant. Using a murine model of the D1-DMBA-3 mammary adenocarcinoma, we demonstrated the unique production of CCL2 by splenic T lymphocytes from tumor-bearing animals. Because this tumor produces GM-CSF, and this factor is also up-regulated in the B lymphocytes of tumor-bearing mice, we looked at the ability of GM-CSF to induce CCL2 production by T cells. Treatment of normal and tumor bearers' T cells with GM-CSF resulted in an increased secretion of this chemokine. This up-regulation was seen with or without stimulation by Concanavalin A, although these treatments were additive in their effects. The induction of CCL2 was studied at the molecular level by analyzing the effect(s) of a variety of physiological and pharmacological agents on cultured T cells. These results suggest that the tumor-derived factor GM-CSF activates various signaling pathways within splenic T cells to up-regulate CCL2 expression.

AB - MCP-1/CCL2 (monocyte chemoattractant protein-1/CC chemokine ligand 2) is a β or CC chemokine that is expressed by a variety of cell types, including fibroblasts, endothelial, smooth muscle, and glial cells. In addition, cells involved in immunity, such as monocytes/macrophages, neutrophils, and eosinophils have also been shown to express this chemoattractant. Using a murine model of the D1-DMBA-3 mammary adenocarcinoma, we demonstrated the unique production of CCL2 by splenic T lymphocytes from tumor-bearing animals. Because this tumor produces GM-CSF, and this factor is also up-regulated in the B lymphocytes of tumor-bearing mice, we looked at the ability of GM-CSF to induce CCL2 production by T cells. Treatment of normal and tumor bearers' T cells with GM-CSF resulted in an increased secretion of this chemokine. This up-regulation was seen with or without stimulation by Concanavalin A, although these treatments were additive in their effects. The induction of CCL2 was studied at the molecular level by analyzing the effect(s) of a variety of physiological and pharmacological agents on cultured T cells. These results suggest that the tumor-derived factor GM-CSF activates various signaling pathways within splenic T cells to up-regulate CCL2 expression.

KW - CCL2

KW - GM-CSF

KW - Mammary tumor

KW - T lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=34548104710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548104710&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 129

EP - 136

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 1

ER -