Glyphosate-based herbicide induces hyperplastic ducts in the mammary gland of aging Wistar rats

María V. Zanardi, Marlise Guerrero Schimpf, María P. Gastiazoro, María M. Milesi, Mónica Muñoz-de-Toro, Jorgelina Varayoud, Milena Durando

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Glyphosate-based herbicide (GBH) exposure is known to have adverse effects on endocrine-related tissues. Here, we aimed to determine whether early postnatal exposure to a GBH induces long-term effects on the rat mammary gland. Thus, female Wistar pups were injected with saline solution (Control) or GBH (2 mg glyphosate/kg/day) on postnatal days (PND) 1, 3, 5 and 7. At 20 months of age, mammary gland samples were collected to determine histomorphological features, proliferation index and the expression of steroid hormone receptors expression, by immunohistochemistry, and serum samples were collected to assess 17β-estradiol (E2) and progesterone (P4) levels. GBH exposure induced morphological changes evidenced by a higher percentage of hyperplastic ducts and a fibroblastic-like stroma in the mammary gland. GBH-treated rats also showed a high expression of steroid hormone receptors in hyperplastic ducts. The results indicate that early postnatal exposure to GBH induces long-term alterations in the mammary gland morphology of aging female rats.

Original languageEnglish (US)
Article number110658
JournalMolecular and Cellular Endocrinology
StatePublished - Feb 5 2020
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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