Abstract
Purpose. The juxtacanalicular tissue (JCT) is the probable site of aqueous outflow resistance in normal eyes and of the increased resistance in primary open-angle glaucoma eyes (POAG). The purpose of this histochemical study was to determine the glycosaminoglycan (GAG) composition and stratification in the JCT of POAG and age-matched normal eyes. Methods. Five eyes from four normal donors and five eyes from four POAG donors (69 to 80 years of age) were analyzed. Using methods that histochemically preserve GAGs, GAG-degrading enzymes, Alcian blue staining, and real color discrimination to exclude pigment, nuclear staining and unstained areas, the type and amount of GAGs were estimated by computer-aided charge-coupled device color video image analysis. To examine GAG stratification, the JCT was segmented into three regions-anterior, middle, and posterior-to examine regional differences in GAG composition: each region was further divided into four 2-μm layers, from layer 1, adjacent to and including the endothelium of Schlemm's canal, to layer 4, to the first trabecular lamellae. Results. The normal GAG JCT profile was as follows: hyaluronic acid (HA), 7.78 ± 1.23 femtograms (fg)/μm2; chondroitin sulfates (CS), 8.18 ± 0.82 fg/μm2; dermatan sulfate, 0.29 ± 0.18 fg/μm2; the total, 18.73 ± 0.68 fg/μm2. In contrast, the POAG GAG JCT profile was as follows: HA 0.57 ± 0.31 fg/μm2 (P < 0.00001), a 93% decrease; CS 13.49 ± 0.74 fg/μm2 (P < 0.0001), a 83% increase; dermatan sulfate, 0.90 ± 0.53 fg/μm2, and the total, 17.31 ± 0.95 fg/μm2, an 8.2% decrease. The HA was depleted in all layers of all regions of POAG JCT. Conclusions. Results indicate that the normal JCT is stratified, with HA as the predominant GAG in layers 1 and 2. The POAG JCT is depleted of HA and has an accumulation of CS, which may increase outflow resistance and, consequently, increase intraocular pressure in patients with POAG.
| Original language | English |
|---|---|
| Pages (from-to) | 2414-2425 |
| Number of pages | 12 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 12 |
| State | Published - Nov 1 1996 |
Fingerprint
Keywords
- chondrotin sulfate
- glycosaminoglycans
- hyaluronic acid
- trabecular meshwork
ASJC Scopus subject areas
- Ophthalmology
Cite this
Glycosaminoglycan stratification of the juxtacanalicular tissue in normal and primary open-angle glaucoma. / Knepper, Paul A.; Goossens, William; Palmberg, Paul.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 12, 01.11.1996, p. 2414-2425.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycosaminoglycan stratification of the juxtacanalicular tissue in normal and primary open-angle glaucoma
AU - Knepper, Paul A.
AU - Goossens, William
AU - Palmberg, Paul
PY - 1996/11/1
Y1 - 1996/11/1
N2 - Purpose. The juxtacanalicular tissue (JCT) is the probable site of aqueous outflow resistance in normal eyes and of the increased resistance in primary open-angle glaucoma eyes (POAG). The purpose of this histochemical study was to determine the glycosaminoglycan (GAG) composition and stratification in the JCT of POAG and age-matched normal eyes. Methods. Five eyes from four normal donors and five eyes from four POAG donors (69 to 80 years of age) were analyzed. Using methods that histochemically preserve GAGs, GAG-degrading enzymes, Alcian blue staining, and real color discrimination to exclude pigment, nuclear staining and unstained areas, the type and amount of GAGs were estimated by computer-aided charge-coupled device color video image analysis. To examine GAG stratification, the JCT was segmented into three regions-anterior, middle, and posterior-to examine regional differences in GAG composition: each region was further divided into four 2-μm layers, from layer 1, adjacent to and including the endothelium of Schlemm's canal, to layer 4, to the first trabecular lamellae. Results. The normal GAG JCT profile was as follows: hyaluronic acid (HA), 7.78 ± 1.23 femtograms (fg)/μm2; chondroitin sulfates (CS), 8.18 ± 0.82 fg/μm2; dermatan sulfate, 0.29 ± 0.18 fg/μm2; the total, 18.73 ± 0.68 fg/μm2. In contrast, the POAG GAG JCT profile was as follows: HA 0.57 ± 0.31 fg/μm2 (P < 0.00001), a 93% decrease; CS 13.49 ± 0.74 fg/μm2 (P < 0.0001), a 83% increase; dermatan sulfate, 0.90 ± 0.53 fg/μm2, and the total, 17.31 ± 0.95 fg/μm2, an 8.2% decrease. The HA was depleted in all layers of all regions of POAG JCT. Conclusions. Results indicate that the normal JCT is stratified, with HA as the predominant GAG in layers 1 and 2. The POAG JCT is depleted of HA and has an accumulation of CS, which may increase outflow resistance and, consequently, increase intraocular pressure in patients with POAG.
AB - Purpose. The juxtacanalicular tissue (JCT) is the probable site of aqueous outflow resistance in normal eyes and of the increased resistance in primary open-angle glaucoma eyes (POAG). The purpose of this histochemical study was to determine the glycosaminoglycan (GAG) composition and stratification in the JCT of POAG and age-matched normal eyes. Methods. Five eyes from four normal donors and five eyes from four POAG donors (69 to 80 years of age) were analyzed. Using methods that histochemically preserve GAGs, GAG-degrading enzymes, Alcian blue staining, and real color discrimination to exclude pigment, nuclear staining and unstained areas, the type and amount of GAGs were estimated by computer-aided charge-coupled device color video image analysis. To examine GAG stratification, the JCT was segmented into three regions-anterior, middle, and posterior-to examine regional differences in GAG composition: each region was further divided into four 2-μm layers, from layer 1, adjacent to and including the endothelium of Schlemm's canal, to layer 4, to the first trabecular lamellae. Results. The normal GAG JCT profile was as follows: hyaluronic acid (HA), 7.78 ± 1.23 femtograms (fg)/μm2; chondroitin sulfates (CS), 8.18 ± 0.82 fg/μm2; dermatan sulfate, 0.29 ± 0.18 fg/μm2; the total, 18.73 ± 0.68 fg/μm2. In contrast, the POAG GAG JCT profile was as follows: HA 0.57 ± 0.31 fg/μm2 (P < 0.00001), a 93% decrease; CS 13.49 ± 0.74 fg/μm2 (P < 0.0001), a 83% increase; dermatan sulfate, 0.90 ± 0.53 fg/μm2, and the total, 17.31 ± 0.95 fg/μm2, an 8.2% decrease. The HA was depleted in all layers of all regions of POAG JCT. Conclusions. Results indicate that the normal JCT is stratified, with HA as the predominant GAG in layers 1 and 2. The POAG JCT is depleted of HA and has an accumulation of CS, which may increase outflow resistance and, consequently, increase intraocular pressure in patients with POAG.
KW - chondrotin sulfate
KW - glycosaminoglycans
KW - hyaluronic acid
KW - trabecular meshwork
UR - http://www.scopus.com/inward/record.url?scp=0029801486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029801486&partnerID=8YFLogxK
M3 - Article
C2 - 8933758
AN - SCOPUS:0029801486
VL - 37
SP - 2414
EP - 2425
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 12
ER -