Studies were directed toward defining relationships between brain ion transport, glycolysis, and oxidative phosphorylation. This was done by examining the relative sensitivity to hypoxemia and to iodoacetate (lAA)-induced inhibition of glycolysis in rats anesthetized with pentobarbital. Both insults had minimal effects on Ko+ baseline. In response to neuronal activation, IAA increased the time required for Ko+ clearance from maximal values to half-recovery of baseline. Hypoxemia slowed the later phase of Ko+ clearance, when Ko+ was approaching "resting" levels. Hypoxemia produced greater declines in high-energy intermediates than did IAA, which indicated that the IAA effect was not due to a greater overall insult to metabolism and suggested a direct link between ATP produced by glycolysis and ion transport activity. These data demonstrate that Ko+ clearance requires energy from glycolysis and oxidative phosphorylation for different phases of the recovery process and that inhibition specific to glycolysis or oxidative phosphorylation may be temporally resolved within a single stimulus.
- Ion transport hypoxemia
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism