Glycogen contains phosphodiester groups that can be introduced by UDPglucose:glycogen glucose 1-phosphotransferase

Joseph Lomako, Wieslawa M. Lomako, William J. Whelan, Richard B. Marchase

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Rabbit-muscle glycogen contains covalently bound phosphorus, equivalent to 1 phosphate group per 208 glucose residues. This often disputed, minor component was previously thought to represent a phosphomonoester group at C-6 of a glucose residue. Here we show that more than half the phosphorus is present as a phosphodiester, the remainder being monoester. A novel enzyme activity has been found in muscle that can account for the presence of the phosphodiester in glycogen. This is a UDPglucose:glycogen glucose 1 -phosphotransferase that positions glucose 1 -phosphate on C-6 of glucose residues in glycogen, forming a diester. The phosphomonoester groups present may arise by removal of the glucose residue originally transferred as glucose 1-phosphate.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalFEBS letters
Volume329
Issue number3
DOIs
StatePublished - Aug 30 1993

Keywords

  • Glucose 1-phosphate transferase
  • Glycogen
  • Glycogen phosphodiester

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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