Glycogen biogenesis in the adipocyte and mammary adenocarcinoma and the influence of insulin

J. Lomako, W. M. Lomako, W. J. Whelan

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3T3 LI P-3 adipocytes and a rat mammary carcinoma each contain a protein (or proteins) of Mr 400 kDa approx. that undergoes glucosylation when incubated with UDP-glucose and Mn2+. In each case, the product of glucosylation is a protein-bound maltosaccharide containing up to 14 glucose residues, and therefore capable of priming glycogen synthesis. The proteins bear a formal similarity to the self-glycosylating protein of Mr 37 kDa that primes muscle glycogen synthesis, but do not cross-react with an antibody that cross-reacts with the muscle protein. The extent of protein glucosylation in the adipocyte is rapidly and markedly stimulated when the cells are bathed in insulin, suggesting that this initial step in glycogen biogenesis, protein glucosylation, is under the influence of the hormone.

Original languageEnglish (US)
Pages (from-to)507-517
Number of pages11
JournalBiochemistry International
Issue number3
StatePublished - Aug 23 1990


ASJC Scopus subject areas

  • Biochemistry

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