TY - JOUR
T1 - Glutathione ester protects against hydroxynonenal-induced loss of auditory hair cells
AU - Ruiz, Jose W.
AU - Guzman, Jose
AU - Polak, Marek
AU - Eshraghi, Adrien A.
AU - Balkany, Thomas J.
AU - Van De Water, Thomas R.
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Objective: Test the ability of glutathione monoethyl ester (GSHe) to protect auditory hair cells against the ototoxic effects of 4-hydroxy-2,3-nonenal (HNE). Study design and setting: Organ of Corti explants were either untreated or treated with one of a series of four concentrations of GSHe for one day, then exposed to HNE. Counts of FITC-phalloidin-labeled hair cells determined both HNE ototoxicity and GSHe otoprotection. Results: HNE was toxic to hair cells at physiologically relevant levels, eg, 400 μM, and GSHe provided a significant level of protection against HNE ototoxicity (P < 0.05) at all levels tested, ie, 1.16 to 9.3 mM. Conclusion: GSHe protects auditory hair cells from damage and loss initiated by a naturally occurring ototoxic molecule, ie, HNE (a by-product of oxidative stress). Significance: Treatment with GSHe may be an effective therapy to protect the cochlea against the adverse effects of traumas (eg, electrode insertion) that generate oxidative stress.
AB - Objective: Test the ability of glutathione monoethyl ester (GSHe) to protect auditory hair cells against the ototoxic effects of 4-hydroxy-2,3-nonenal (HNE). Study design and setting: Organ of Corti explants were either untreated or treated with one of a series of four concentrations of GSHe for one day, then exposed to HNE. Counts of FITC-phalloidin-labeled hair cells determined both HNE ototoxicity and GSHe otoprotection. Results: HNE was toxic to hair cells at physiologically relevant levels, eg, 400 μM, and GSHe provided a significant level of protection against HNE ototoxicity (P < 0.05) at all levels tested, ie, 1.16 to 9.3 mM. Conclusion: GSHe protects auditory hair cells from damage and loss initiated by a naturally occurring ototoxic molecule, ie, HNE (a by-product of oxidative stress). Significance: Treatment with GSHe may be an effective therapy to protect the cochlea against the adverse effects of traumas (eg, electrode insertion) that generate oxidative stress.
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U2 - 10.1016/j.otohns.2006.05.031
DO - 10.1016/j.otohns.2006.05.031
M3 - Article
C2 - 17071314
AN - SCOPUS:33750368887
VL - 135
SP - 792
EP - 797
JO - Otolaryngology - Head and Neck Surgery (United States)
JF - Otolaryngology - Head and Neck Surgery (United States)
SN - 0194-5998
IS - 5
ER -