Glutamine uptake by neurons: Interaction of protons with system a transporters

Farrukh A. Chaudhry, Dietmar Schmitz, Richard J. Reimer, Hans P Larsson, Andrew T. Gray, Roger Nicoll, Michael Kavanaugh, Robert H. Edwards

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Astrocytes provide the glutamine required by neurons to synthesize glutamate and GABA. However, the mechanisms involved in glutamine transfer from glia to neurons have remained poorly understood. Recent work has implicated the System N transporter SN1 in the efflux of glutamine from astrocytes and the very closely related System A transporters SA1 and SA2 in glutamine uptake by neurons. To understand how these closely related proteins mediate flux in different directions, we have examined their ionic coupling. In contrast to the electroneutral exchange of H+ for Na+ and neutral amino acid catalyzed by SN1, we now show that SA1 and SA2 do not couple H+ movement to amino acid flux. As a result, SA1 and SA2 are electrogenic and do not mediate flux reversal as readily as SN1. Differences between System N and A transporters in coupling to H+ thus contribute to the delivery of glutamine from glia to neurons. Nonetheless, although they are not transported, H+ inhibit SA1 and SA2 by competing with Na+.

Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalJournal of Neuroscience
Volume22
Issue number1
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Glutamine
Protons
Amino Acid Transport System A
Neurons
Neuroglia
Astrocytes
Neutral Amino Acids
gamma-Aminobutyric Acid
Glutamic Acid
Amino Acids
Proteins

Keywords

  • Glutamine
  • Glutamine-glutamate cycle
  • H coupling
  • Synaptic transmission
  • System A
  • System N

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chaudhry, F. A., Schmitz, D., Reimer, R. J., Larsson, H. P., Gray, A. T., Nicoll, R., ... Edwards, R. H. (2002). Glutamine uptake by neurons: Interaction of protons with system a transporters. Journal of Neuroscience, 22(1), 62-72.

Glutamine uptake by neurons : Interaction of protons with system a transporters. / Chaudhry, Farrukh A.; Schmitz, Dietmar; Reimer, Richard J.; Larsson, Hans P; Gray, Andrew T.; Nicoll, Roger; Kavanaugh, Michael; Edwards, Robert H.

In: Journal of Neuroscience, Vol. 22, No. 1, 01.01.2002, p. 62-72.

Research output: Contribution to journalArticle

Chaudhry, FA, Schmitz, D, Reimer, RJ, Larsson, HP, Gray, AT, Nicoll, R, Kavanaugh, M & Edwards, RH 2002, 'Glutamine uptake by neurons: Interaction of protons with system a transporters', Journal of Neuroscience, vol. 22, no. 1, pp. 62-72.
Chaudhry FA, Schmitz D, Reimer RJ, Larsson HP, Gray AT, Nicoll R et al. Glutamine uptake by neurons: Interaction of protons with system a transporters. Journal of Neuroscience. 2002 Jan 1;22(1):62-72.
Chaudhry, Farrukh A. ; Schmitz, Dietmar ; Reimer, Richard J. ; Larsson, Hans P ; Gray, Andrew T. ; Nicoll, Roger ; Kavanaugh, Michael ; Edwards, Robert H. / Glutamine uptake by neurons : Interaction of protons with system a transporters. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 1. pp. 62-72.
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