Glucose-stimulated efflux of indo-1 from pancreatic β-cells is reduced by probenecid

Per Arkhammar, Thomas Nilsson, Per Olof Berggren

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Indo-1 loaded pancreatic β-cells, isolated from obese hyperglycaemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), efflux of indicator and insulin release. In the absence of glucose there was a continuous efflux of indo-1 which increased upon stimulation with 20 mM of the sugar. The anion exchange inhibitor probenecid reduced both basal efflux of indo-1 and prevented that promoted by glucose. Measurements of [Ca2+]i and insulin release revealed similar results as previously reported with quin-2 and fura-2. Furthermore, probenecid did not influence the [Ca2+]i responses. It is thus possible to reduce efflux of indo-1 probenecid and thereby improve the measurements of [Ca2+]iin pancreatic β-cells.

Original languageEnglish (US)
Pages (from-to)182-184
Number of pages3
JournalFEBS letters
Volume273
Issue number1-2
DOIs
StatePublished - Oct 29 1990

Keywords

  • Cytoplasmic free Ca
  • Indo-1
  • Pancreatic β-cell
  • Probenecid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Glucose-stimulated efflux of indo-1 from pancreatic β-cells is reduced by probenecid'. Together they form a unique fingerprint.

  • Cite this