Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid

Per Arkhammar, Thomas Nilsson, Per Olof Berggren

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalBiochemical and biophysical research communications
Volume159
Issue number1
DOIs
StatePublished - Feb 28 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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