TY - JOUR
T1 - Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid
AU - Arkhammar, Per
AU - Nilsson, Thomas
AU - Berggren, Per Olof
N1 - Funding Information:
This work was supported by grants from the Swedish Medical Research Council (12x-562, 12x-109 and 19x-00034), the Bank of Sweden Tercentenary Foundation, the Medical Faculty of Uppsala University, the Swedish Diabetes Association, Novo Industry, Swedish Hoechst, the Nordic Insulin, Clas Groschinskys, Tore Nilsons, Aage Louis-Hansens, Amundsons, the Family Ernfors and Magnus Bergvalls Foundations. We thank Dr Rolf Sammann, Swedish Hoechst, for the generous gift of 1251-labelled insulin.
PY - 1989/2/28
Y1 - 1989/2/28
N2 - Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.
AB - Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.
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U2 - 10.1016/0006-291X(89)92426-1
DO - 10.1016/0006-291X(89)92426-1
M3 - Article
C2 - 2647080
AN - SCOPUS:0024519053
VL - 159
SP - 223
EP - 228
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -