Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid

Per Arkhammar; Thomas Nilsson; Per Olof Berggren

doi:10.1016/0006-291X(89)92426-1

Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid

Per Arkhammar, Thomas Nilsson, Per Olof Berggren

Surgery

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21 Scopus citations

Abstract

Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca²⁺ concentration ([Ca²⁺]_i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca²⁺]_i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca²⁺]_i.

Original language	English (US)
Pages (from-to)	223-228
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)92426-1
State	Published - Feb 28 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid",

abstract = "Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.",

author = "Per Arkhammar and Thomas Nilsson and Berggren, {Per Olof}",

note = "Funding Information: This work was supported by grants from the Swedish Medical Research Council (12x-562, 12x-109 and 19x-00034), the Bank of Sweden Tercentenary Foundation, the Medical Faculty of Uppsala University, the Swedish Diabetes Association, Novo Industry, Swedish Hoechst, the Nordic Insulin, Clas Groschinskys, Tore Nilsons, Aage Louis-Hansens, Amundsons, the Family Ernfors and Magnus Bergvalls Foundations. We thank Dr Rolf Sammann, Swedish Hoechst, for the generous gift of 1251-labelled insulin.",

year = "1989",

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doi = "10.1016/0006-291X(89)92426-1",

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T1 - Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid

AU - Arkhammar, Per

AU - Nilsson, Thomas

AU - Berggren, Per Olof

N1 - Funding Information: This work was supported by grants from the Swedish Medical Research Council (12x-562, 12x-109 and 19x-00034), the Bank of Sweden Tercentenary Foundation, the Medical Faculty of Uppsala University, the Swedish Diabetes Association, Novo Industry, Swedish Hoechst, the Nordic Insulin, Clas Groschinskys, Tore Nilsons, Aage Louis-Hansens, Amundsons, the Family Ernfors and Magnus Bergvalls Foundations. We thank Dr Rolf Sammann, Swedish Hoechst, for the generous gift of 1251-labelled insulin.

PY - 1989/2/28

Y1 - 1989/2/28

N2 - Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.

AB - Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic β-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.

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