Glucose Recruits KATP Channels via Non-Insulin-Containing Dense-Core Granules

Shao Nian Yang, Nancy Dekki Wenna, Jia Yu, Guang Yang, Hua Qiu, Lina Yu, Lisa Juntti-Berggren, Martin Köhler, Per Olof Berggren

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

β cells rely on adenosine triphosphate-sensitive potassium (KATP) channels to initiate and end glucose-stimulated insulin secretion through changes in membrane potential. These channels may also act as a constituent of the exocytotic machinery to mediate insulin release independent of their electrical function. However, the molecular mechanisms whereby the β cell plasma membrane maintains an appropriate number of KATP channels are not known. We now show that glucose increases KATP current amplitude by increasing the number of KATP channels in the β cell plasma membrane. The effect was blocked by inhibition of protein kinase A (PKA) as well as by depletion of extracellular or intracellular Ca2+. Furthermore, glucose promoted recruitment of the potassium inward rectifier 6.2 to the plasma membrane, and intracellular KATP channels localized in chromogranin-positive/insulin-negative dense-core granules. Our data suggest that glucose can recruit KATP channels to the β cell plasma membrane via non-insulin-containing dense-core granules in a Ca2+- and PKA-dependent manner.

Original languageEnglish
Pages (from-to)217-228
Number of pages12
JournalCell Metabolism
Volume6
Issue number3
DOIs
StatePublished - Sep 5 2007
Externally publishedYes

Fingerprint

KATP Channels
Cell Membrane
Glucose
Insulin
Cyclic AMP-Dependent Protein Kinases
Chromogranins
Potassium Channels
Membrane Potentials
Potassium
Adenosine Triphosphate

Keywords

  • HUMDISEASE
  • PROTEINS

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Yang, S. N., Wenna, N. D., Yu, J., Yang, G., Qiu, H., Yu, L., ... Berggren, P. O. (2007). Glucose Recruits KATP Channels via Non-Insulin-Containing Dense-Core Granules. Cell Metabolism, 6(3), 217-228. https://doi.org/10.1016/j.cmet.2007.08.002

Glucose Recruits KATP Channels via Non-Insulin-Containing Dense-Core Granules. / Yang, Shao Nian; Wenna, Nancy Dekki; Yu, Jia; Yang, Guang; Qiu, Hua; Yu, Lina; Juntti-Berggren, Lisa; Köhler, Martin; Berggren, Per Olof.

In: Cell Metabolism, Vol. 6, No. 3, 05.09.2007, p. 217-228.

Research output: Contribution to journalArticle

Yang, SN, Wenna, ND, Yu, J, Yang, G, Qiu, H, Yu, L, Juntti-Berggren, L, Köhler, M & Berggren, PO 2007, 'Glucose Recruits KATP Channels via Non-Insulin-Containing Dense-Core Granules', Cell Metabolism, vol. 6, no. 3, pp. 217-228. https://doi.org/10.1016/j.cmet.2007.08.002
Yang, Shao Nian ; Wenna, Nancy Dekki ; Yu, Jia ; Yang, Guang ; Qiu, Hua ; Yu, Lina ; Juntti-Berggren, Lisa ; Köhler, Martin ; Berggren, Per Olof. / Glucose Recruits KATP Channels via Non-Insulin-Containing Dense-Core Granules. In: Cell Metabolism. 2007 ; Vol. 6, No. 3. pp. 217-228.
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