Glucose-induced Oscillations in Cytoplasmic Free Ca2+ Concentration Precede Oscillations in Mitochondrial Membrane Potential in the Pancreatic β-Cell

Henrik Kindmark, Martin Köhler, Graham Brown, Robert Bränström, Olof Larsson, Per Olof Berggren

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Using dual excitation and fixed emission fluorescence microscopy, we were able to measure changes in cytoplasmic free Ca2+ concentration ([Ca2+]i) and mitochondrial membrane potential simultaneously in the pancreatic β-cell. The β-cells were exposed to a combination of the Ca2+ indicator fura-2/AM and the indicator of mitochondrial membrane potential, rhodamine 123 (Rh123). Using simultaneous measurements of mitochondrial membrane potential and [Ca2+] i during glucose stimulation, it was possible to measure the time lag between the onset of mitochondrial hyperpolarization and changes in [Ca 2+]i. Glucose-induced oscillations in [Ca 2+]i were followed by transient depolarizations of mitochondrial membrane potential. These results are compatible with a model in which nadirs in [Ca2+]i oscillations are generated by a transient, Ca2+-induced inhibition of mitochondrial metabolism resulting in a temporary fall in the cytoplasmic ATP/ADP ratio, opening of plasma membrane KATP channels, repolarization of the plasma membrane, and thus transient closure of voltage-gated L-type Ca2+ channels.

Original languageEnglish
Pages (from-to)34530-34536
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number37
DOIs
StatePublished - Sep 14 2001
Externally publishedYes

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Mitochondrial Membrane Potential
Membranes
Glucose
Cell membranes
Cell Membrane
Rhodamine 123
Enzyme inhibition
KATP Channels
Fura-2
Fluorescence microscopy
Depolarization
Ion Channels
Fluorescence Microscopy
Metabolism
Adenosine Diphosphate
Adenosine Triphosphate
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glucose-induced Oscillations in Cytoplasmic Free Ca2+ Concentration Precede Oscillations in Mitochondrial Membrane Potential in the Pancreatic β-Cell. / Kindmark, Henrik; Köhler, Martin; Brown, Graham; Bränström, Robert; Larsson, Olof; Berggren, Per Olof.

In: Journal of Biological Chemistry, Vol. 276, No. 37, 14.09.2001, p. 34530-34536.

Research output: Contribution to journalArticle

Kindmark, Henrik ; Köhler, Martin ; Brown, Graham ; Bränström, Robert ; Larsson, Olof ; Berggren, Per Olof. / Glucose-induced Oscillations in Cytoplasmic Free Ca2+ Concentration Precede Oscillations in Mitochondrial Membrane Potential in the Pancreatic β-Cell. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 37. pp. 34530-34536.
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