Glucose-induced increase in cytoplasmic pH in pancreatic β-cells is mediated by Na+/H+ exchange, an effect not dependent on protein kinase C

Lisa Juntti-Berggren, Per Arkhammar, Thomas Nilsson, Patrik Rorsman, Per Olof Berggren

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Abstract

Glucose-induced changes in cytoplasmic pH (pHi) were investigated using pancreatic β-cells isolated from obese hyperglycemic mice. Glucose, at concentrations above 3-5 mM, depolarized the β-cell and increased pHi, cytoplasmic free Ca2+ ([Ca2+]i), and insulin release. This increase in pHi was dependent on the presence of extracellular Na+ and was inhibited by 5-(N-ethyl-N-isopropyl) amiloride, a blocker of Na+/H+ exchange. Stimulation of protein kinase C with phorbol ester also induced an alkalinization. However, when protein kinase C activity was down-regulated, glucose stimulation still induced alkalinization. At 20 mM glucose, 10 mM NH4Cl induced a marked rise in pHi, paralleled by repolarization, inhibition of electrical activity, and decreases in both [Ca2+]i and insulin release. Reduction in [Ca2+]i was prevented by 200 μM tolbutamide, but not by 10 mM tetraethylammonium. At 4 mM glucose, NH4Cl induced a transient increase in insulin release, without changing [Ca2+]i. Exposure of β-cells to 10 mM sodium acetate caused a persistent decrease in pHi, an effect paralleled by a small transient increase in [Ca2+]i. Acidification per se did not change the β-cell sensitivity to glucose, not excluding that the activity of the ATP-regulated K+ channels may be modulated by changes in pHi.

Original languageEnglish
Pages (from-to)23537-23541
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number35
StatePublished - Dec 15 1991
Externally publishedYes

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Protein Kinase C
Glucose
Obese Mice
Insulin
Sodium Acetate
Tolbutamide
Tetraethylammonium
Acidification
Phorbol Esters
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glucose-induced increase in cytoplasmic pH in pancreatic β-cells is mediated by Na+/H+ exchange, an effect not dependent on protein kinase C. / Juntti-Berggren, Lisa; Arkhammar, Per; Nilsson, Thomas; Rorsman, Patrik; Berggren, Per Olof.

In: Journal of Biological Chemistry, Vol. 266, No. 35, 15.12.1991, p. 23537-23541.

Research output: Contribution to journalArticle

Juntti-Berggren, L, Arkhammar, P, Nilsson, T, Rorsman, P & Berggren, PO 1991, 'Glucose-induced increase in cytoplasmic pH in pancreatic β-cells is mediated by Na+/H+ exchange, an effect not dependent on protein kinase C', Journal of Biological Chemistry, vol. 266, no. 35, pp. 23537-23541.
Juntti-Berggren, Lisa ; Arkhammar, Per ; Nilsson, Thomas ; Rorsman, Patrik ; Berggren, Per Olof. / Glucose-induced increase in cytoplasmic pH in pancreatic β-cells is mediated by Na+/H+ exchange, an effect not dependent on protein kinase C. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 35. pp. 23537-23541.
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