Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice

A. Khan, V. Chandramouli, C. G. Östenson, P. O. Berggren, H. Löw, B. R. Landau, S. Efendic

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Pancreatic islets from fed 7-month old lean and obese hyperglycemic mice (ob/ob) were incubated with 3H2O and 5.5 mM or 16.7 mM glucose. Incorporation of 3H into the medium glucose was taken as the measure of glucose-6-P hydrolysis to glucose. Glucose utilization was measured from the yield of 3H2O from [5-3H]glucose. Only 3-4% of the glucose phosphorylated was dephosphorylated by the lean mouse islets irrespective of the glucose concentration. In contrast, the ob/ob mouse islets at 5.5 mM glucose dephosphorylated 18% of the glucose phosphorylated and 30% at 16.7 mM. Thus, the islets of hyperglycemic mice demonstrate increased glucose cycling as compared to the islets of normoglycemic lean mice.

Original languageEnglish (US)
Pages (from-to)2413-2416
Number of pages4
JournalEndocrinology
Volume126
Issue number5
DOIs
StatePublished - May 1990

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice'. Together they form a unique fingerprint.

  • Cite this

    Khan, A., Chandramouli, V., Östenson, C. G., Berggren, P. O., Löw, H., Landau, B. R., & Efendic, S. (1990). Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice. Endocrinology, 126(5), 2413-2416. https://doi.org/10.1210/endo-126-5-2413