Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice

A. Khan, V. Chandramouli, C. G. Östenson, P. O. Berggren, H. Löw, B. R. Landau, S. Efendic

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Abstract

Pancreatic islets from fed 7-month old lean and obese hyperglycemic mice (ob/ob) were incubated with 3H2O and 5.5 mM or 16.7 mM glucose. Incorporation of 3H into the medium glucose was taken as the measure of glucose-6-P hydrolysis to glucose. Glucose utilization was measured from the yield of 3H2O from [5-3H]glucose. Only 3-4% of the glucose phosphorylated was dephosphorylated by the lean mouse islets irrespective of the glucose concentration. In contrast, the ob/ob mouse islets at 5.5 mM glucose dephosphorylated 18% of the glucose phosphorylated and 30% at 16.7 mM. Thus, the islets of hyperglycemic mice demonstrate increased glucose cycling as compared to the islets of normoglycemic lean mice.

Original languageEnglish
Pages (from-to)2413-2416
Number of pages4
JournalEndocrinology
Volume126
Issue number5
StatePublished - May 1 1990
Externally publishedYes

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Obese Mice
Islets of Langerhans
Glucose
Hydrolysis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Khan, A., Chandramouli, V., Östenson, C. G., Berggren, P. O., Löw, H., Landau, B. R., & Efendic, S. (1990). Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice. Endocrinology, 126(5), 2413-2416.

Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice. / Khan, A.; Chandramouli, V.; Östenson, C. G.; Berggren, P. O.; Löw, H.; Landau, B. R.; Efendic, S.

In: Endocrinology, Vol. 126, No. 5, 01.05.1990, p. 2413-2416.

Research output: Contribution to journalArticle

Khan, A, Chandramouli, V, Östenson, CG, Berggren, PO, Löw, H, Landau, BR & Efendic, S 1990, 'Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice', Endocrinology, vol. 126, no. 5, pp. 2413-2416.
Khan A, Chandramouli V, Östenson CG, Berggren PO, Löw H, Landau BR et al. Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice. Endocrinology. 1990 May 1;126(5):2413-2416.
Khan, A. ; Chandramouli, V. ; Östenson, C. G. ; Berggren, P. O. ; Löw, H. ; Landau, B. R. ; Efendic, S. / Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice. In: Endocrinology. 1990 ; Vol. 126, No. 5. pp. 2413-2416.
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