Glucocorticoids stimulate the production of preprocalcitonin-derived secretory peptides by a rat medullary thyroid carcinoma cell line

M. Muszynski, R. S. Birnbaum, B. A. Roos

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Abstract

A rat medullary thyroid carcinoma cell line, CA-77, has been established as a model system for investigating calcitonin biosynthesis and secretion. Growth of this cell line in serum-free defined medium provided suitable conditions for studying steroid hormone effects on the production of calcitonin and related peptides. After exposure for 5 days to a variety of steroids, only dexamethasone and corticosterone increased cellular content of calcitonin and a second secretory peptide (CCAP) derived from the same mRNA translation product as calcitonin. Glucocorticoids had no effect on cellular somatostatin, another secretory product of these cells. Increasing doses of dexamethasone progressively elevated cellular calcitonin and CCAP, with a maximal effect at 10-8 M; 10-9 M and lower doses were ineffective. On a molar basis, corticosterone was approximately 50-fold less potent than the synthetic glucocorticoid. An increase in cellular calcitonin content was observed only after 48 h of glucocorticoid treatment; a maximum increase (13-fold) occurred after 7 days. Glucocorticoids also increased basal calcitonin secretion. Similar effects were observed for cellular and secreted CCAP. Withdrawal of dexamethasone after 4 days of treatment lowered cellular calcitonin toward the level of control cultures. Dexamethasone pretreatment potentiated the acute secretory response to calcium for both calcitonin and CCAP, while no such enhancement was noted for calcium stimulation of somatostatin secretion. We conclude that the glucocorticoids specifically stimulate the production and secretion of calcitonin and CCAP, two secretory peptides derived from preprocalcitonin.

Original languageEnglish
Pages (from-to)11678-11683
Number of pages6
JournalJournal of Biological Chemistry
Volume258
Issue number19
StatePublished - Jan 1 1983
Externally publishedYes

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Calcitonin
Glucocorticoids
Rats
Cells
Cell Line
Peptides
Dexamethasone
Corticosterone
Somatostatin
Steroid hormones
Steroids
preprocalcitonin
Medullary Thyroid cancer
Calcium
Biosynthesis
Serum-Free Culture Media
Protein Biosynthesis
katacalcin
Hormones
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glucocorticoids stimulate the production of preprocalcitonin-derived secretory peptides by a rat medullary thyroid carcinoma cell line. / Muszynski, M.; Birnbaum, R. S.; Roos, B. A.

In: Journal of Biological Chemistry, Vol. 258, No. 19, 01.01.1983, p. 11678-11683.

Research output: Contribution to journalArticle

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