Glucocorticoid treatment at moderate doses of SIVmac251-infected rhesus macaques decreases the frequency of circulating CD14+CD16++ monocytes but does not alter the tissue virus reservoir

Marcin Moniuszko, Namal P.M. Liyanage, Melvin N. Doster, Robyn Washington Parks, Kamil Grubczak, Danuta Lipinska, Katherine Mckinnon, Charles Brown, Vanessa Hirsch, Monica Vaccari, Shari Gordon, Poonam Pegu, Claudio Fenizia, Robert Flisiak, Anna Grzeszczuk, Milena Dabrowska, Marjorie Robert-Guroff, Guido Silvestri, Mario Stevenson, Joseph MccuneGenoveffa Franchini

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Subsets of CD16-positive monocytes produce proinflammatory cytokines and expand during chronic infection with the human immunodeficiency virus type 1 (HIV). HIV-infected macrophage in tissues may be long lived and contribute to the establishment and maintenance of the HIV reservoir. We found that the (intermediate) CD14++CD16+ and (nonclassical) CD14+CD16++ monocyte subsets are significantly expanded during infection of Rhesus macaques with pathogenic SIVmac251 but not during infection of sooty mangabeys with the nonpathogenic isolate SIVSM. In vitro glucocorticoid (GC) treatment of peripheral blood mononuclear cells (PBMCs) from uninfected or SIVmac251-infected Rhesus macaques and HIV-infected patients treated or not with antiretroviral therapy (ART) resulted in a significant decrease in the frequency of both CD16-positive monocyte subsets. Short-term in vivo treatment with high doses of GC of chronically SIVmac251-infected macaques resulted in a significant decrease in the CD14+CD16++ population and, to a lesser extent, in the CD14++CD16+ monocytes, as well as a significant decrease in the number of macrophages in tissues. Surprisingly, treatment of SIVmac251-infected macaques with ART significantly increased the CD14++CD16+ population and the addition of GC resulted in a significant decrease in only the CD14+CD16++ subset. No difference in SIV DNA levels in blood, lymph nodes, gut, and spleen was found between the groups treated with ART or ART plus GC. Thus, it appears that high doses of GC treatment in the absence of ART could affect both CD16-positive populations in vivo. Whether the efficacy of this treatment at higher doses to decrease virus levels outweighs its risks remains to be determined.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalAIDS research and human retroviruses
Issue number1
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases


Dive into the research topics of 'Glucocorticoid treatment at moderate doses of SIV<sub>mac251</sub>-infected rhesus macaques decreases the frequency of circulating CD14<sup>+</sup>CD16<sup>++</sup> monocytes but does not alter the tissue virus reservoir'. Together they form a unique fingerprint.

Cite this