Glucocorticoid treatment at moderate doses of SIV<inf>mac251</inf>-infected rhesus macaques decreases the frequency of circulating CD14<sup>+</sup>CD16<sup>++</sup> monocytes but does not alter the tissue virus reservoir

Marcin Moniuszko, Namal P M Liyanage, Melvin N. Doster, Robyn Washington Parks, Kamil Grubczak, Danuta Lipinska, Katherine Mckinnon, Charles Brown, Vanessa Hirsch, Monica Vaccari, Shari Gordon, Poonam Pegu, Claudio Fenizia, Robert Flisiak, Anna Grzeszczuk, Milena Dabrowska, Marjorie Robert-Guroff, Guido Silvestri, Mario Stevenson, Joseph MccuneGenoveffa Franchini

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Subsets of CD16-positive monocytes produce proinflammatory cytokines and expand during chronic infection with the human immunodeficiency virus type 1 (HIV). HIV-infected macrophage in tissues may be long lived and contribute to the establishment and maintenance of the HIV reservoir. We found that the (intermediate) CD14<sup>++</sup>CD16<sup>+</sup> and (nonclassical) CD14<sup>+</sup>CD16<sup>++</sup> monocyte subsets are significantly expanded during infection of Rhesus macaques with pathogenic SIV<inf>mac251</inf> but not during infection of sooty mangabeys with the nonpathogenic isolate SIV<inf>SM</inf>. In vitro glucocorticoid (GC) treatment of peripheral blood mononuclear cells (PBMCs) from uninfected or SIV<inf>mac251</inf>-infected Rhesus macaques and HIV-infected patients treated or not with antiretroviral therapy (ART) resulted in a significant decrease in the frequency of both CD16-positive monocyte subsets. Short-term in vivo treatment with high doses of GC of chronically SIV<inf>mac251</inf>-infected macaques resulted in a significant decrease in the CD14<sup>+</sup>CD16<sup>++</sup> population and, to a lesser extent, in the CD14<sup>++</sup>CD16<sup>+</sup> monocytes, as well as a significant decrease in the number of macrophages in tissues. Surprisingly, treatment of SIV<inf>mac251</inf>-infected macaques with ART significantly increased the CD14<sup>++</sup>CD16<sup>+</sup> population and the addition of GC resulted in a significant decrease in only the CD14<sup>+</sup>CD16<sup>++</sup> subset. No difference in SIV DNA levels in blood, lymph nodes, gut, and spleen was found between the groups treated with ART or ART plus GC. Thus, it appears that high doses of GC treatment in the absence of ART could affect both CD16-positive populations in vivo. Whether the efficacy of this treatment at higher doses to decrease virus levels outweighs its risks remains to be determined.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalAIDS Research and Human Retroviruses
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Macaca mulatta
Glucocorticoids
Monocytes
Viruses
HIV-1
Therapeutics
Macaca
Cercocebus atys
Infection
Macrophages
Population
Blood Cells
Spleen
Lymph Nodes
Maintenance
Cytokines
DNA

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Glucocorticoid treatment at moderate doses of SIV<inf>mac251</inf>-infected rhesus macaques decreases the frequency of circulating CD14<sup>+</sup>CD16<sup>++</sup> monocytes but does not alter the tissue virus reservoir. / Moniuszko, Marcin; Liyanage, Namal P M; Doster, Melvin N.; Parks, Robyn Washington; Grubczak, Kamil; Lipinska, Danuta; Mckinnon, Katherine; Brown, Charles; Hirsch, Vanessa; Vaccari, Monica; Gordon, Shari; Pegu, Poonam; Fenizia, Claudio; Flisiak, Robert; Grzeszczuk, Anna; Dabrowska, Milena; Robert-Guroff, Marjorie; Silvestri, Guido; Stevenson, Mario; Mccune, Joseph; Franchini, Genoveffa.

In: AIDS Research and Human Retroviruses, Vol. 31, No. 1, 01.01.2015, p. 115-126.

Research output: Contribution to journalArticle

Moniuszko, M, Liyanage, NPM, Doster, MN, Parks, RW, Grubczak, K, Lipinska, D, Mckinnon, K, Brown, C, Hirsch, V, Vaccari, M, Gordon, S, Pegu, P, Fenizia, C, Flisiak, R, Grzeszczuk, A, Dabrowska, M, Robert-Guroff, M, Silvestri, G, Stevenson, M, Mccune, J & Franchini, G 2015, 'Glucocorticoid treatment at moderate doses of SIV<inf>mac251</inf>-infected rhesus macaques decreases the frequency of circulating CD14<sup>+</sup>CD16<sup>++</sup> monocytes but does not alter the tissue virus reservoir', AIDS Research and Human Retroviruses, vol. 31, no. 1, pp. 115-126. https://doi.org/10.1089/aid.2013.0220
Moniuszko, Marcin ; Liyanage, Namal P M ; Doster, Melvin N. ; Parks, Robyn Washington ; Grubczak, Kamil ; Lipinska, Danuta ; Mckinnon, Katherine ; Brown, Charles ; Hirsch, Vanessa ; Vaccari, Monica ; Gordon, Shari ; Pegu, Poonam ; Fenizia, Claudio ; Flisiak, Robert ; Grzeszczuk, Anna ; Dabrowska, Milena ; Robert-Guroff, Marjorie ; Silvestri, Guido ; Stevenson, Mario ; Mccune, Joseph ; Franchini, Genoveffa. / Glucocorticoid treatment at moderate doses of SIV<inf>mac251</inf>-infected rhesus macaques decreases the frequency of circulating CD14<sup>+</sup>CD16<sup>++</sup> monocytes but does not alter the tissue virus reservoir. In: AIDS Research and Human Retroviruses. 2015 ; Vol. 31, No. 1. pp. 115-126.
@article{f2ef85b5163747518eb5f95023b5ce24,
title = "Glucocorticoid treatment at moderate doses of SIVmac251-infected rhesus macaques decreases the frequency of circulating CD14+CD16++ monocytes but does not alter the tissue virus reservoir",
abstract = "Subsets of CD16-positive monocytes produce proinflammatory cytokines and expand during chronic infection with the human immunodeficiency virus type 1 (HIV). HIV-infected macrophage in tissues may be long lived and contribute to the establishment and maintenance of the HIV reservoir. We found that the (intermediate) CD14++CD16+ and (nonclassical) CD14+CD16++ monocyte subsets are significantly expanded during infection of Rhesus macaques with pathogenic SIVmac251 but not during infection of sooty mangabeys with the nonpathogenic isolate SIVSM. In vitro glucocorticoid (GC) treatment of peripheral blood mononuclear cells (PBMCs) from uninfected or SIVmac251-infected Rhesus macaques and HIV-infected patients treated or not with antiretroviral therapy (ART) resulted in a significant decrease in the frequency of both CD16-positive monocyte subsets. Short-term in vivo treatment with high doses of GC of chronically SIVmac251-infected macaques resulted in a significant decrease in the CD14+CD16++ population and, to a lesser extent, in the CD14++CD16+ monocytes, as well as a significant decrease in the number of macrophages in tissues. Surprisingly, treatment of SIVmac251-infected macaques with ART significantly increased the CD14++CD16+ population and the addition of GC resulted in a significant decrease in only the CD14+CD16++ subset. No difference in SIV DNA levels in blood, lymph nodes, gut, and spleen was found between the groups treated with ART or ART plus GC. Thus, it appears that high doses of GC treatment in the absence of ART could affect both CD16-positive populations in vivo. Whether the efficacy of this treatment at higher doses to decrease virus levels outweighs its risks remains to be determined.",
author = "Marcin Moniuszko and Liyanage, {Namal P M} and Doster, {Melvin N.} and Parks, {Robyn Washington} and Kamil Grubczak and Danuta Lipinska and Katherine Mckinnon and Charles Brown and Vanessa Hirsch and Monica Vaccari and Shari Gordon and Poonam Pegu and Claudio Fenizia and Robert Flisiak and Anna Grzeszczuk and Milena Dabrowska and Marjorie Robert-Guroff and Guido Silvestri and Mario Stevenson and Joseph Mccune and Genoveffa Franchini",
year = "2015",
month = "1",
day = "1",
doi = "10.1089/aid.2013.0220",
language = "English (US)",
volume = "31",
pages = "115--126",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Glucocorticoid treatment at moderate doses of SIVmac251-infected rhesus macaques decreases the frequency of circulating CD14+CD16++ monocytes but does not alter the tissue virus reservoir

AU - Moniuszko, Marcin

AU - Liyanage, Namal P M

AU - Doster, Melvin N.

AU - Parks, Robyn Washington

AU - Grubczak, Kamil

AU - Lipinska, Danuta

AU - Mckinnon, Katherine

AU - Brown, Charles

AU - Hirsch, Vanessa

AU - Vaccari, Monica

AU - Gordon, Shari

AU - Pegu, Poonam

AU - Fenizia, Claudio

AU - Flisiak, Robert

AU - Grzeszczuk, Anna

AU - Dabrowska, Milena

AU - Robert-Guroff, Marjorie

AU - Silvestri, Guido

AU - Stevenson, Mario

AU - Mccune, Joseph

AU - Franchini, Genoveffa

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Subsets of CD16-positive monocytes produce proinflammatory cytokines and expand during chronic infection with the human immunodeficiency virus type 1 (HIV). HIV-infected macrophage in tissues may be long lived and contribute to the establishment and maintenance of the HIV reservoir. We found that the (intermediate) CD14++CD16+ and (nonclassical) CD14+CD16++ monocyte subsets are significantly expanded during infection of Rhesus macaques with pathogenic SIVmac251 but not during infection of sooty mangabeys with the nonpathogenic isolate SIVSM. In vitro glucocorticoid (GC) treatment of peripheral blood mononuclear cells (PBMCs) from uninfected or SIVmac251-infected Rhesus macaques and HIV-infected patients treated or not with antiretroviral therapy (ART) resulted in a significant decrease in the frequency of both CD16-positive monocyte subsets. Short-term in vivo treatment with high doses of GC of chronically SIVmac251-infected macaques resulted in a significant decrease in the CD14+CD16++ population and, to a lesser extent, in the CD14++CD16+ monocytes, as well as a significant decrease in the number of macrophages in tissues. Surprisingly, treatment of SIVmac251-infected macaques with ART significantly increased the CD14++CD16+ population and the addition of GC resulted in a significant decrease in only the CD14+CD16++ subset. No difference in SIV DNA levels in blood, lymph nodes, gut, and spleen was found between the groups treated with ART or ART plus GC. Thus, it appears that high doses of GC treatment in the absence of ART could affect both CD16-positive populations in vivo. Whether the efficacy of this treatment at higher doses to decrease virus levels outweighs its risks remains to be determined.

AB - Subsets of CD16-positive monocytes produce proinflammatory cytokines and expand during chronic infection with the human immunodeficiency virus type 1 (HIV). HIV-infected macrophage in tissues may be long lived and contribute to the establishment and maintenance of the HIV reservoir. We found that the (intermediate) CD14++CD16+ and (nonclassical) CD14+CD16++ monocyte subsets are significantly expanded during infection of Rhesus macaques with pathogenic SIVmac251 but not during infection of sooty mangabeys with the nonpathogenic isolate SIVSM. In vitro glucocorticoid (GC) treatment of peripheral blood mononuclear cells (PBMCs) from uninfected or SIVmac251-infected Rhesus macaques and HIV-infected patients treated or not with antiretroviral therapy (ART) resulted in a significant decrease in the frequency of both CD16-positive monocyte subsets. Short-term in vivo treatment with high doses of GC of chronically SIVmac251-infected macaques resulted in a significant decrease in the CD14+CD16++ population and, to a lesser extent, in the CD14++CD16+ monocytes, as well as a significant decrease in the number of macrophages in tissues. Surprisingly, treatment of SIVmac251-infected macaques with ART significantly increased the CD14++CD16+ population and the addition of GC resulted in a significant decrease in only the CD14+CD16++ subset. No difference in SIV DNA levels in blood, lymph nodes, gut, and spleen was found between the groups treated with ART or ART plus GC. Thus, it appears that high doses of GC treatment in the absence of ART could affect both CD16-positive populations in vivo. Whether the efficacy of this treatment at higher doses to decrease virus levels outweighs its risks remains to be determined.

UR - http://www.scopus.com/inward/record.url?scp=84929948140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929948140&partnerID=8YFLogxK

U2 - 10.1089/aid.2013.0220

DO - 10.1089/aid.2013.0220

M3 - Article

C2 - 24432835

AN - SCOPUS:84929948140

VL - 31

SP - 115

EP - 126

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 1

ER -