Glucocorticoid regulation of amidating enzyme in a neoplastic C-cell line

Roger S. Birnbaum, Arthur H. Bertelsen, Bernard A. Roos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Posttranslationar carboxyl-terminal amidation of many peptides is accomplished by peptidylglycine α-amidating monooxygenase. We have previously demonstrated that glucocorticoids stimulate production of amidated products by the CA-77 rat medullary thyroid carcinoma cell line. The present investigation was undertaken to determine whether amidation enzyme activity changes in parallel. Enzyme activity, similar to that found in other tissues, was readily detected in cell extracts and conditioned cultured medium. Stimulation with the calcitonin secretagogue calcium increased secretion of enzyme activity and lowered cell extract activity. Treatment of cultures with dexamethasone, but no other steroid, decreased by 50-70% the basal amidation enzyme activity secreted. There was no associated change in cellular activity. The decrease in medium activity was partially reversible and steroid-dose dependent. The glucocorticoid-induced change in medium activity was due to a decreased Vm. These experiments demonstrate that the a-amidating activity of the CA-77 cells can be hormonally regulated.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume61
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Enzyme activity
Glucocorticoids
Cells
Cell Line
Enzymes
Cell Extracts
Steroids
Calcitonin
Conditioned Culture Medium
Mixed Function Oxygenases
Dexamethasone
Rats
Tissue
Calcium
Peptides
Experiments

Keywords

  • Amidation enzyme
  • C cell
  • Posttranslational processing enzyme

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Glucocorticoid regulation of amidating enzyme in a neoplastic C-cell line. / Birnbaum, Roger S.; Bertelsen, Arthur H.; Roos, Bernard A.

In: Molecular and Cellular Endocrinology, Vol. 61, No. 1, 01.01.1989, p. 109-116.

Research output: Contribution to journalArticle

Birnbaum, Roger S. ; Bertelsen, Arthur H. ; Roos, Bernard A. / Glucocorticoid regulation of amidating enzyme in a neoplastic C-cell line. In: Molecular and Cellular Endocrinology. 1989 ; Vol. 61, No. 1. pp. 109-116.
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