Glucocorticoid receptors in purified subpopulations of human peripheral blood lymphocytes

Marc E Lippman, R. Barr

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

On the premise that the differential effects of glucocorticoids on various aspects of the immune response may be mediated by differences in the glucocorticoid receptors in the effector cells, subpopulations of human peripheral blood lymphocytes were examined for these receptors as well as for glucocorticoid responsiveness. Purified T and non T lymphocytes, when studied by a sensitive whole cell assay technique, contained equivalent amounts of specific glucocorticoid receptor, which, by binding affinity and specificity measurements, were indistinguishable from each other. Furthermore, under in vitro incubation conditions, macromolecular synthesis in both of these cell populations was inhibited by glucocorticoid at concentrations which saturated the receptor sites. It is concluded that the putative differential effects of glucocorticoids on T and non T lymphocyte associated functions are probably not mediated by differences in the glucocorticoid receptors in these cell populations.

Original languageEnglish
Pages (from-to)1977-1981
Number of pages5
JournalJournal of Immunology
Volume118
Issue number6
StatePublished - Dec 1 1977
Externally publishedYes

Fingerprint

Glucocorticoid Receptors
Glucocorticoids
Lymphocytes
T-Lymphocytes
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Glucocorticoid receptors in purified subpopulations of human peripheral blood lymphocytes. / Lippman, Marc E; Barr, R.

In: Journal of Immunology, Vol. 118, No. 6, 01.12.1977, p. 1977-1981.

Research output: Contribution to journalArticle

Lippman, Marc E ; Barr, R. / Glucocorticoid receptors in purified subpopulations of human peripheral blood lymphocytes. In: Journal of Immunology. 1977 ; Vol. 118, No. 6. pp. 1977-1981.
@article{cfce1bec013b40679ccb71557d91eb9f,
title = "Glucocorticoid receptors in purified subpopulations of human peripheral blood lymphocytes",
abstract = "On the premise that the differential effects of glucocorticoids on various aspects of the immune response may be mediated by differences in the glucocorticoid receptors in the effector cells, subpopulations of human peripheral blood lymphocytes were examined for these receptors as well as for glucocorticoid responsiveness. Purified T and non T lymphocytes, when studied by a sensitive whole cell assay technique, contained equivalent amounts of specific glucocorticoid receptor, which, by binding affinity and specificity measurements, were indistinguishable from each other. Furthermore, under in vitro incubation conditions, macromolecular synthesis in both of these cell populations was inhibited by glucocorticoid at concentrations which saturated the receptor sites. It is concluded that the putative differential effects of glucocorticoids on T and non T lymphocyte associated functions are probably not mediated by differences in the glucocorticoid receptors in these cell populations.",
author = "Lippman, {Marc E} and R. Barr",
year = "1977",
month = "12",
day = "1",
language = "English",
volume = "118",
pages = "1977--1981",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Glucocorticoid receptors in purified subpopulations of human peripheral blood lymphocytes

AU - Lippman, Marc E

AU - Barr, R.

PY - 1977/12/1

Y1 - 1977/12/1

N2 - On the premise that the differential effects of glucocorticoids on various aspects of the immune response may be mediated by differences in the glucocorticoid receptors in the effector cells, subpopulations of human peripheral blood lymphocytes were examined for these receptors as well as for glucocorticoid responsiveness. Purified T and non T lymphocytes, when studied by a sensitive whole cell assay technique, contained equivalent amounts of specific glucocorticoid receptor, which, by binding affinity and specificity measurements, were indistinguishable from each other. Furthermore, under in vitro incubation conditions, macromolecular synthesis in both of these cell populations was inhibited by glucocorticoid at concentrations which saturated the receptor sites. It is concluded that the putative differential effects of glucocorticoids on T and non T lymphocyte associated functions are probably not mediated by differences in the glucocorticoid receptors in these cell populations.

AB - On the premise that the differential effects of glucocorticoids on various aspects of the immune response may be mediated by differences in the glucocorticoid receptors in the effector cells, subpopulations of human peripheral blood lymphocytes were examined for these receptors as well as for glucocorticoid responsiveness. Purified T and non T lymphocytes, when studied by a sensitive whole cell assay technique, contained equivalent amounts of specific glucocorticoid receptor, which, by binding affinity and specificity measurements, were indistinguishable from each other. Furthermore, under in vitro incubation conditions, macromolecular synthesis in both of these cell populations was inhibited by glucocorticoid at concentrations which saturated the receptor sites. It is concluded that the putative differential effects of glucocorticoids on T and non T lymphocyte associated functions are probably not mediated by differences in the glucocorticoid receptors in these cell populations.

UR - http://www.scopus.com/inward/record.url?scp=0017684712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017684712&partnerID=8YFLogxK

M3 - Article

C2 - 301151

AN - SCOPUS:0017684712

VL - 118

SP - 1977

EP - 1981

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -