Abstract
Steroid binding activity (SBP) in glucocorticoid sensitive and resistant lymphoblasts was measured by a competitive binding assay in which cytosol was incubated at 0°C in the presence of various concentrations of 3H dexamethasone in the presence or absence of an excess of unlabeled dexamethasone, modified by performing the reaction for 2 hr in buffer which had been made 20% with respect to glycerol. The authors have demonstrated a class of highly specific, saturable, glucocorticoid receptor molecules in cytosol preparations, obtained from glucocorticoid sensitive human ALL cells. There is a positive correlation between steroid responsiveness of lymphoblasts and the presence of this SBP and a lack of glucocorticoid responsiveness in its absence. An in vitro test of steroid responsiveness might help leukemia treatment both by identifying those patients likely to benefit from steroid therapy and eliminating use of glucocorticoids when leukemic cells are no longer inhibited by steroids, thus avoiding unnecessary drug toxicity.
| Original language | English |
|---|---|
| Title of host publication | NATURE NEW BIOL. |
| Pages | 157-158 |
| Number of pages | 2 |
| Volume | 242 |
| Edition | 118 |
| State | Published - Dec 1 1973 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Glucocorticoid binding proteins in human leukaemic lymphoblasts. / Lippman, Marc E; Halterman, R.; Perry, S.; Leventhal, B.; Thompson, E. B.
NATURE NEW BIOL.. Vol. 242 118. ed. 1973. p. 157-158.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Glucocorticoid binding proteins in human leukaemic lymphoblasts
AU - Lippman, Marc E
AU - Halterman, R.
AU - Perry, S.
AU - Leventhal, B.
AU - Thompson, E. B.
PY - 1973/12/1
Y1 - 1973/12/1
N2 - Steroid binding activity (SBP) in glucocorticoid sensitive and resistant lymphoblasts was measured by a competitive binding assay in which cytosol was incubated at 0°C in the presence of various concentrations of 3H dexamethasone in the presence or absence of an excess of unlabeled dexamethasone, modified by performing the reaction for 2 hr in buffer which had been made 20% with respect to glycerol. The authors have demonstrated a class of highly specific, saturable, glucocorticoid receptor molecules in cytosol preparations, obtained from glucocorticoid sensitive human ALL cells. There is a positive correlation between steroid responsiveness of lymphoblasts and the presence of this SBP and a lack of glucocorticoid responsiveness in its absence. An in vitro test of steroid responsiveness might help leukemia treatment both by identifying those patients likely to benefit from steroid therapy and eliminating use of glucocorticoids when leukemic cells are no longer inhibited by steroids, thus avoiding unnecessary drug toxicity.
AB - Steroid binding activity (SBP) in glucocorticoid sensitive and resistant lymphoblasts was measured by a competitive binding assay in which cytosol was incubated at 0°C in the presence of various concentrations of 3H dexamethasone in the presence or absence of an excess of unlabeled dexamethasone, modified by performing the reaction for 2 hr in buffer which had been made 20% with respect to glycerol. The authors have demonstrated a class of highly specific, saturable, glucocorticoid receptor molecules in cytosol preparations, obtained from glucocorticoid sensitive human ALL cells. There is a positive correlation between steroid responsiveness of lymphoblasts and the presence of this SBP and a lack of glucocorticoid responsiveness in its absence. An in vitro test of steroid responsiveness might help leukemia treatment both by identifying those patients likely to benefit from steroid therapy and eliminating use of glucocorticoids when leukemic cells are no longer inhibited by steroids, thus avoiding unnecessary drug toxicity.
UR - http://www.scopus.com/inward/record.url?scp=0015843720&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015843720&partnerID=8YFLogxK
M3 - Chapter
C2 - 4349567
AN - SCOPUS:0015843720
VL - 242
SP - 157
EP - 158
BT - NATURE NEW BIOL.
ER -