TY - JOUR
T1 - GLP-1 mediated improvement of the glucose tolerance in the T2DM GK rat model after massive jejunal resection
AU - Prada-Oliveira, J. Arturo
AU - Camacho-Ramirez, Alonso
AU - Salas-Alvarez, Jesús
AU - Campos-Martinez, Francisco Javier
AU - Lechuga-Sancho, Alfonso M.
AU - Almorza-Gomar, David
AU - Blandino-Rosano, Manuel
AU - Perez-Arana, Gonzalo M.
N1 - Funding Information:
This work was partially supported by the Research Vicerrectorado of the University of Cádiz. Ref. 20INPR0484.
Publisher Copyright:
© 2019 Elsevier GmbH
PY - 2019/5
Y1 - 2019/5
N2 - Objective: The aim of this study was to clarify the role of the middle gut in the entero-pancreatic axis modification that leads to glucose improvement in the Goto-Kakizaki (GK) rat as a non-obese T2DM model. Background: Bariatric surgery is considered an assured solution for type 2 Diabetes (T2DM). Enterohormones such as ghrelin, gastric inhibitory polypeptide and mainly glucagon-like peptide-1 (GLP-1) were recognized as key players in the physiophathological mechanisms associated with entero-pancreatic axis regulation and glucose tolerance improvement. However, the influence of anatomical arrangements post-bariatric surgery on this axis is still debatable. Method: To this purpose, 50% of small intestine resections were performed on GK rats (n = 6), preserving the proximal half of the jejunum and the ileum (IR50). Phenotypic and functional changes, such as performance in oral glucose tolerance tests, ileal release of GLP-1, beta-cell sensitivity to GLP-1, beta-cell mass, and turnover were characterized in IR50 and the surgical control group (Sham). Results: The glucose tolerance was improved and ileal release of GLP-1 was enhanced four weeks after IR50 versus the control group rats. Beta-cell mass, beta-cell proliferation, and beta-cell sensitivity to GLP-1 were also increased in the pancreas of IR50 versus the control group rats. Conclusion: the jejunal exclusion increases beta-cell-mass and improves glucose tolerance by increasing in GLP-1 expression and number of receptors via the entero-pancreatic axis.
AB - Objective: The aim of this study was to clarify the role of the middle gut in the entero-pancreatic axis modification that leads to glucose improvement in the Goto-Kakizaki (GK) rat as a non-obese T2DM model. Background: Bariatric surgery is considered an assured solution for type 2 Diabetes (T2DM). Enterohormones such as ghrelin, gastric inhibitory polypeptide and mainly glucagon-like peptide-1 (GLP-1) were recognized as key players in the physiophathological mechanisms associated with entero-pancreatic axis regulation and glucose tolerance improvement. However, the influence of anatomical arrangements post-bariatric surgery on this axis is still debatable. Method: To this purpose, 50% of small intestine resections were performed on GK rats (n = 6), preserving the proximal half of the jejunum and the ileum (IR50). Phenotypic and functional changes, such as performance in oral glucose tolerance tests, ileal release of GLP-1, beta-cell sensitivity to GLP-1, beta-cell mass, and turnover were characterized in IR50 and the surgical control group (Sham). Results: The glucose tolerance was improved and ileal release of GLP-1 was enhanced four weeks after IR50 versus the control group rats. Beta-cell mass, beta-cell proliferation, and beta-cell sensitivity to GLP-1 were also increased in the pancreas of IR50 versus the control group rats. Conclusion: the jejunal exclusion increases beta-cell-mass and improves glucose tolerance by increasing in GLP-1 expression and number of receptors via the entero-pancreatic axis.
KW - Enterohormones
KW - GLP-1
KW - Insulin-Secreting Cells
KW - Malabsorption Syndromes
KW - type 2 Diabetes mellitus
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U2 - 10.1016/j.aanat.2019.01.007
DO - 10.1016/j.aanat.2019.01.007
M3 - Article
C2 - 30721719
AN - SCOPUS:85061232477
VL - 223
SP - 1
EP - 7
JO - Annals of Anatomy
JF - Annals of Anatomy
SN - 0940-9602
ER -