Glomerulosclerosis at both early and late stages is associated with increased cell turnover in mice transgenic for growth hormone

C. M. Pesce, L. J. Striker, E. Peten, Sharon Elliot, G. E. Striker

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The evolution of glomerulosclerosis consists of a progressive increase in mesangial matrix with gradual glomerular obliteration. The sclerotic process is thought to be irreversible and include a progressive loss of glomerular cells. To investigate this process, we selected mice transgenic for bovine growth hormone because they develop progressive glomerulosclerosis and renal failure. The sequence of histologic events in the growth hormone mice consists initially of an increase in the number of centrolobular glomerular cells, followed by an accumulation of extracellular matrix. This is accompanied by an increase in glomerular size which is disproportionate to the overall increment in kidney or body weight. The [3H]thymidine labeling index of the cells of the glomerular tuft was assessed before the development of recognizable sclerosis and at a time when the sclerosis was far advanced. The labeling index was more than five-fold increased over controls at the early time point. Contrary to what was expected, the labeling index remained at the same high levels in densely sclerotic glomeruli at the late time point. In conclusion, increased cell turnover is a significant component of the sclerotic process both at the onset and in the late stages of this model.

Original languageEnglish
Pages (from-to)601-605
Number of pages5
JournalLaboratory Investigation
Volume65
Issue number5
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Transgenic Mice
Growth Hormone
Sclerosis
Thymidine
Renal Insufficiency
Extracellular Matrix
Body Weight
Kidney

Keywords

  • Autoradiography

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Glomerulosclerosis at both early and late stages is associated with increased cell turnover in mice transgenic for growth hormone. / Pesce, C. M.; Striker, L. J.; Peten, E.; Elliot, Sharon; Striker, G. E.

In: Laboratory Investigation, Vol. 65, No. 5, 01.12.1991, p. 601-605.

Research output: Contribution to journalArticle

@article{d00c345a6a4a4adeae1653186c2b2b98,
title = "Glomerulosclerosis at both early and late stages is associated with increased cell turnover in mice transgenic for growth hormone",
abstract = "The evolution of glomerulosclerosis consists of a progressive increase in mesangial matrix with gradual glomerular obliteration. The sclerotic process is thought to be irreversible and include a progressive loss of glomerular cells. To investigate this process, we selected mice transgenic for bovine growth hormone because they develop progressive glomerulosclerosis and renal failure. The sequence of histologic events in the growth hormone mice consists initially of an increase in the number of centrolobular glomerular cells, followed by an accumulation of extracellular matrix. This is accompanied by an increase in glomerular size which is disproportionate to the overall increment in kidney or body weight. The [3H]thymidine labeling index of the cells of the glomerular tuft was assessed before the development of recognizable sclerosis and at a time when the sclerosis was far advanced. The labeling index was more than five-fold increased over controls at the early time point. Contrary to what was expected, the labeling index remained at the same high levels in densely sclerotic glomeruli at the late time point. In conclusion, increased cell turnover is a significant component of the sclerotic process both at the onset and in the late stages of this model.",
keywords = "Autoradiography",
author = "Pesce, {C. M.} and Striker, {L. J.} and E. Peten and Sharon Elliot and Striker, {G. E.}",
year = "1991",
month = "12",
day = "1",
language = "English",
volume = "65",
pages = "601--605",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Glomerulosclerosis at both early and late stages is associated with increased cell turnover in mice transgenic for growth hormone

AU - Pesce, C. M.

AU - Striker, L. J.

AU - Peten, E.

AU - Elliot, Sharon

AU - Striker, G. E.

PY - 1991/12/1

Y1 - 1991/12/1

N2 - The evolution of glomerulosclerosis consists of a progressive increase in mesangial matrix with gradual glomerular obliteration. The sclerotic process is thought to be irreversible and include a progressive loss of glomerular cells. To investigate this process, we selected mice transgenic for bovine growth hormone because they develop progressive glomerulosclerosis and renal failure. The sequence of histologic events in the growth hormone mice consists initially of an increase in the number of centrolobular glomerular cells, followed by an accumulation of extracellular matrix. This is accompanied by an increase in glomerular size which is disproportionate to the overall increment in kidney or body weight. The [3H]thymidine labeling index of the cells of the glomerular tuft was assessed before the development of recognizable sclerosis and at a time when the sclerosis was far advanced. The labeling index was more than five-fold increased over controls at the early time point. Contrary to what was expected, the labeling index remained at the same high levels in densely sclerotic glomeruli at the late time point. In conclusion, increased cell turnover is a significant component of the sclerotic process both at the onset and in the late stages of this model.

AB - The evolution of glomerulosclerosis consists of a progressive increase in mesangial matrix with gradual glomerular obliteration. The sclerotic process is thought to be irreversible and include a progressive loss of glomerular cells. To investigate this process, we selected mice transgenic for bovine growth hormone because they develop progressive glomerulosclerosis and renal failure. The sequence of histologic events in the growth hormone mice consists initially of an increase in the number of centrolobular glomerular cells, followed by an accumulation of extracellular matrix. This is accompanied by an increase in glomerular size which is disproportionate to the overall increment in kidney or body weight. The [3H]thymidine labeling index of the cells of the glomerular tuft was assessed before the development of recognizable sclerosis and at a time when the sclerosis was far advanced. The labeling index was more than five-fold increased over controls at the early time point. Contrary to what was expected, the labeling index remained at the same high levels in densely sclerotic glomeruli at the late time point. In conclusion, increased cell turnover is a significant component of the sclerotic process both at the onset and in the late stages of this model.

KW - Autoradiography

UR - http://www.scopus.com/inward/record.url?scp=0026332780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026332780&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 601

EP - 605

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 5

ER -