Global transcriptional analysis of Streptococcus mutans sugar transporters using microarrays

Dragana Ajdic, Vi T T Pham

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The transport of carbohydrates by Streptococcus mutans is accomplished by the phosphoenolpyruvate-phosphotransferase system (PTS) and ATP-binding cassette (ABC) transporters. To undertake a global transcriptional analysis of all S. mutans sugar transporters simultaneously, we used a whole-genome expression microarray. Global transcription profiles of S. mutans UA159 were determined for several monosaccharides (glucose, fructose, galactose, and mannose), disaccharides (sucrose, lactose, maltose, and trehalose), a β-glucoside (cellobiose), oligosaccharides (raffinose, stachyose, and maltotriose), and a sugar alcohol (mannitol). The results revealed that PTSs were responsible for transport of monosaccharides, disaccharides, β-glucosides, and sugar alcohol. Six PTSs were transcribed only if a specific sugar was present in the growth medium; thus, they were regulated at the transcriptional level. These included transporters for fructose, lactose, cellobiose, and trehalose and two transporters for mannitol. Three PTSs were repressed under all conditions tested. Interestingly, five PTSs were always highly expressed regardless of the sugar source used, presumably suggesting their availability for immediate uptake of most common dietary sugars (glucose, fructose, maltose, and sucrose). The ABC transporters were found to be specific for oligosaccharides, raffinose, stachyose, and isomaltosaccharides. Compared to the PTSs, the ABC transporters showed higher transcription under several tested conditions, suggesting that they might be transporting multiple substrates.

Original languageEnglish
Pages (from-to)5049-5059
Number of pages11
JournalJournal of Bacteriology
Volume189
Issue number14
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

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Streptococcus mutans
ATP-Binding Cassette Transporters
Fructose
Raffinose
Sugar Alcohols
Cellobiose
Trehalose
Maltose
Monosaccharides
Disaccharides
Glucosides
Mannitol
Lactose
Oligosaccharides
Sucrose
Dietary Sucrose
Glucose
Phosphoenolpyruvate
Mannose
Galactose

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Global transcriptional analysis of Streptococcus mutans sugar transporters using microarrays. / Ajdic, Dragana; Pham, Vi T T.

In: Journal of Bacteriology, Vol. 189, No. 14, 01.07.2007, p. 5049-5059.

Research output: Contribution to journalArticle

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