Abstract
To test the potential for vasoactive neuropeptide receptors to affect capillary resistance, we have begun to study the plausibiIity that pericytes might be equipped to respond to a representative peptide vasoconstrictor and a representative peptide vasodilator. Pericytes cultured from the bovine retinal vasculature specifically bind the angiotensin II (Ang II) antagonist saralasin (1 nM 125I-saralasin bound at 2.2±0.41 fmol/mg protein) and 125I-vasoactive intestinal peptide (VIP; K(d) of 0.5 nM with a population of 30 fmol/mg protein). Incubation with 100 μM Ang II induced minimal cAMP synthesis, while VIP (1 μM, 100 μM) did not induce any change in cAMP concentration. Ang II (10 μM and 100 μM) caused contraction of pericytes cultured on an elastic silicone surface. Circulating or locally produced vasoactive neuropeptides might affect pericyte contractile tone via several intracellular pathways, moderated by indirect effects of these peptides through endothelial stimulation, with the net effect on local blood flow resulting from the effects on arteries and veins as well as capillaries.
| Original language | English |
|---|---|
| Pages (from-to) | 269-275 |
| Number of pages | 7 |
| Journal | Ophthalmologica |
| Volume | 210 |
| Issue number | 5 |
| State | Published - Sep 1 1996 |
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Keywords
- Angiotensin
- Blood flow regulation
- Capillaries
- Cell culture
- Glaucoma
- Pericytes
- Retina
- Vasoactive intestinal peptide
ASJC Scopus subject areas
- Ophthalmology
Cite this
Glaucoma, capillaries and pericytes 3. Peptide hormone binding and influence on pericytes. / Ferrari-Dileo, Gabryleda; Davis, E. Barry; Anderson, Douglas.
In: Ophthalmologica, Vol. 210, No. 5, 01.09.1996, p. 269-275.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glaucoma, capillaries and pericytes 3. Peptide hormone binding and influence on pericytes
AU - Ferrari-Dileo, Gabryleda
AU - Davis, E. Barry
AU - Anderson, Douglas
PY - 1996/9/1
Y1 - 1996/9/1
N2 - To test the potential for vasoactive neuropeptide receptors to affect capillary resistance, we have begun to study the plausibiIity that pericytes might be equipped to respond to a representative peptide vasoconstrictor and a representative peptide vasodilator. Pericytes cultured from the bovine retinal vasculature specifically bind the angiotensin II (Ang II) antagonist saralasin (1 nM 125I-saralasin bound at 2.2±0.41 fmol/mg protein) and 125I-vasoactive intestinal peptide (VIP; K(d) of 0.5 nM with a population of 30 fmol/mg protein). Incubation with 100 μM Ang II induced minimal cAMP synthesis, while VIP (1 μM, 100 μM) did not induce any change in cAMP concentration. Ang II (10 μM and 100 μM) caused contraction of pericytes cultured on an elastic silicone surface. Circulating or locally produced vasoactive neuropeptides might affect pericyte contractile tone via several intracellular pathways, moderated by indirect effects of these peptides through endothelial stimulation, with the net effect on local blood flow resulting from the effects on arteries and veins as well as capillaries.
AB - To test the potential for vasoactive neuropeptide receptors to affect capillary resistance, we have begun to study the plausibiIity that pericytes might be equipped to respond to a representative peptide vasoconstrictor and a representative peptide vasodilator. Pericytes cultured from the bovine retinal vasculature specifically bind the angiotensin II (Ang II) antagonist saralasin (1 nM 125I-saralasin bound at 2.2±0.41 fmol/mg protein) and 125I-vasoactive intestinal peptide (VIP; K(d) of 0.5 nM with a population of 30 fmol/mg protein). Incubation with 100 μM Ang II induced minimal cAMP synthesis, while VIP (1 μM, 100 μM) did not induce any change in cAMP concentration. Ang II (10 μM and 100 μM) caused contraction of pericytes cultured on an elastic silicone surface. Circulating or locally produced vasoactive neuropeptides might affect pericyte contractile tone via several intracellular pathways, moderated by indirect effects of these peptides through endothelial stimulation, with the net effect on local blood flow resulting from the effects on arteries and veins as well as capillaries.
KW - Angiotensin
KW - Blood flow regulation
KW - Capillaries
KW - Cell culture
KW - Glaucoma
KW - Pericytes
KW - Retina
KW - Vasoactive intestinal peptide
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UR - http://www.scopus.com/inward/citedby.url?scp=0029817076&partnerID=8YFLogxK
M3 - Article
C2 - 8878209
AN - SCOPUS:0029817076
VL - 210
SP - 269
EP - 275
JO - Ophthalmologica
JF - Ophthalmologica
SN - 0030-3755
IS - 5
ER -