Germinal center-specific protein human germinal center associated lymphoma directly interacts with both myosin and actin and increases the binding of myosin to actin

Xiaoqing Lu, Katarzyna Kazmierczak, Xiaoyu Jiang, Michelle Jones, James Watt, David M. Helfman, Jeffrey R. Moore, Danuta Szczesna-Cordary, Izidore S. Lossos

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Human germinal center associated lymphoma (HGAL) is a germinal center-specific gene whose expression correlates with a favorable prognosis in patients with diffuse large B-cell and classic Hodgkin lymphomas. HGAL is involved in negative regulation of lymphocyte motility. The movement of lymphocytes is directly driven by actin polymerization and actin-myosin interactions. We demonstrate that HGAL interacts directly and independently with both actin and myosin and delineate the HGAL and myosin domains responsible for the interaction. Furthermore, we show that HGAL increases the binding of myosin to F-actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement. No effects of HGAL on actomyosin ATPase activity and the rate of actin polymerization from G-actin to F-actin were observed. These findings reveal a new mechanism underlying the inhibitory effects of germinal center-specific HGAL protein on lymphocyte and lymphoma cell motility. HGAL is a germinal center-specific gene negatively regulating lymphocyte motility. We demonstrate that HGAL interacts directly and independently with actin and myosin and delineates the HGAL and myosin domains responsible for the interaction, and HGAL increases the binding of myosin to F-actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement.

Original languageEnglish (US)
Pages (from-to)1922-1931
Number of pages10
JournalFEBS Journal
Volume278
Issue number11
DOIs
StatePublished - Jun 1 2011

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Keywords

  • actin
  • germinal center
  • HGAL
  • motility
  • myosin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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