TY - JOUR
T1 - Genotypic and phenotypic antibiotic resistance in Staphylococcus epidermidis endophthalmitis
AU - Laura, Diana M.
AU - Scott, Nathan L.
AU - Vanner, Elizabeth A.
AU - Miller, Darlene
AU - Flynn, Harry W.
N1 - Funding Information:
Originally submitted January 7, 2020. Revisions received January 27, 2020. Accepted January 29, 2020. Presented October 10, 2019, at the Ophthalmology Times Retina Research Scholar Honoree Program in San Francisco, CA. Presented July 27, 2019, at the American Society of Retina Specialists Annual Meeting in Chicago, IL. Presented April 30, 2019, at the Association for Research in Vision and Ophthalmology Annual Meeting in Vancouver, BC. Supported in part by the National Institute of Health Center Core Grant P30EY014801 and an unrestricted grant from Research to Prevent Blindness to Bascom Palmer Eye Institute/University of Miami. The authors report no relevant financial disclosures. Address correspondence to Diana M. Laura, MD, 900 NW 17 Street, Miami, FL 33136; email: dml189@med.miami.edu. doi: 10.3928/23258160-20200108-02
Publisher Copyright:
© 2020 Slack Incorporated. All rights reserved.
PY - 2020/5
Y1 - 2020/5
N2 - BACKGROUND AND OBJECTIVE: To evaluate antibiotic resistance patterns in Staphylococcus epidermis endophthalmitis isolates, identify antibiotic resistance genes, and compare this to their phenotypic resistance. MATERIALS AND METHODS: Retrospective case series of S. epidermidis isolates from January 2012 to December 2017. Phenotypic resistance was determined from minimum inhibitory concentration values. Genotypic resistance was determined from DNA microarray. The relationship between these was analyzed using Cohen's kappa and predictive value. RESULTS: Seventy-five isolates were included. More than 60% were resistant to methicillin and erythromycin. Approximately 60% possessed the resistance genes for methicillin and erythromycin, and 25% for clindamycin. There was near perfect agreement between genotype and phenotype for erythromycin and methicillin (kappa = 0.88 and 0.89, respectively). Approximately 98% (P < 0.0001) of isolates with erythromycin and methicillin resistance genes and 90% (P = 0.0006) with clindamycin resistance genes were phenotypically resistant to those antibiotics. None were resistant to vancomycin. CONCLUSION: The relationship between genotypic and phenotypic antibiotic resistance highlights that resistance may be polygenic, and genes may be silent.
AB - BACKGROUND AND OBJECTIVE: To evaluate antibiotic resistance patterns in Staphylococcus epidermis endophthalmitis isolates, identify antibiotic resistance genes, and compare this to their phenotypic resistance. MATERIALS AND METHODS: Retrospective case series of S. epidermidis isolates from January 2012 to December 2017. Phenotypic resistance was determined from minimum inhibitory concentration values. Genotypic resistance was determined from DNA microarray. The relationship between these was analyzed using Cohen's kappa and predictive value. RESULTS: Seventy-five isolates were included. More than 60% were resistant to methicillin and erythromycin. Approximately 60% possessed the resistance genes for methicillin and erythromycin, and 25% for clindamycin. There was near perfect agreement between genotype and phenotype for erythromycin and methicillin (kappa = 0.88 and 0.89, respectively). Approximately 98% (P < 0.0001) of isolates with erythromycin and methicillin resistance genes and 90% (P = 0.0006) with clindamycin resistance genes were phenotypically resistant to those antibiotics. None were resistant to vancomycin. CONCLUSION: The relationship between genotypic and phenotypic antibiotic resistance highlights that resistance may be polygenic, and genes may be silent.
UR - http://www.scopus.com/inward/record.url?scp=85085908577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085908577&partnerID=8YFLogxK
U2 - 10.3928/23258160-20200108-02
DO - 10.3928/23258160-20200108-02
M3 - Article
C2 - 32484896
AN - SCOPUS:85085908577
VL - 51
SP - S13-S16
JO - Ophthalmic Surgery Lasers and Imaging Retina
JF - Ophthalmic Surgery Lasers and Imaging Retina
SN - 2325-8160
IS - 5
ER -