Genotoxicity of [1h]benz [de] isoquinoline-1, 3[2h]dione, 5 amino-2-, [2-(dimethylamino) ethyl] (BIDA) in human lymphocytes

Niramol Savaraj, Jan Liang, Katherine Lu, Lynn G Feun, T. C. Hsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have investigated the genotoxicity of BIDA in cultured human lymphocytes. Lymphocytes were cultured and stimulated with phytohemagglutinin (PHA) for 72 h. Doses of 0.1, 0.25, 0.5, 0.75, and 1 fig/ml of BIDA were added to the culture at 1 h (G2 phase), and 6 h (S/G2 phase) before harvesting. Cells were harvested at the end of the 72-h culture period with 1-h colcemid treatment to accumulate mitosis, and further prepared by standard cytogenetic technique. BIDA induced chromatic type breakages and chromatid exchanges at both 1 h and 6 h. The mean number of breakages per cell was 0, 0.1, 1.0, and 1.7 after treatment with 0.1, 0.25, and 0.75 kg/ml, respectively. At 1 kg/ml, BIDA severely inhibited cell progression and very few mitoses were observed. At 6 h the mean number of breakages per cell was 0.3 at 0.25 fig/ml and 1.2 at 0.5 kg/ml. Very few cells entered mitosis at 0.75 and 1 kg/ml. To study the effect of BIDA on cells in G0 and G1, BIDA (0.75 kg/ml) was added for 1 h to the cultures at the beginning of culture (G0), or 24 h after (G1) culture initiation. Afterward, cells were washed and reincubated in the conditioned medium for 71 or 47 h. No chromosomal aberrations were seen in these experiments. The number of chromatid breaks was minimal (0.1 to 0.4/cell). Our study suggests that BID A induces chromatid type aberrations during G2 and S phases. The absence of chromosome type aberrations in cells treated during G0 and G1 suggests that either BID A has no effect on these cells or that damaged cells fail to progress through S and G2 to reach mitosis.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalCancer Investigation
Volume7
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

amonafide
Lymphocytes
Mitosis
Chromatids
G2 Phase
Ficus
S Phase
Chromosome Aberrations
Demecolcine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Genotoxicity of [1h]benz [de] isoquinoline-1, 3[2h]dione, 5 amino-2-, [2-(dimethylamino) ethyl] (BIDA) in human lymphocytes. / Savaraj, Niramol; Liang, Jan; Lu, Katherine; Feun, Lynn G; Hsu, T. C.

In: Cancer Investigation, Vol. 7, No. 2, 01.01.1989, p. 117-121.

Research output: Contribution to journalArticle

@article{efe0da9861d24ad099d939a1c7d1e17b,
title = "Genotoxicity of [1h]benz [de] isoquinoline-1, 3[2h]dione, 5 amino-2-, [2-(dimethylamino) ethyl] (BIDA) in human lymphocytes",
abstract = "We have investigated the genotoxicity of BIDA in cultured human lymphocytes. Lymphocytes were cultured and stimulated with phytohemagglutinin (PHA) for 72 h. Doses of 0.1, 0.25, 0.5, 0.75, and 1 fig/ml of BIDA were added to the culture at 1 h (G2 phase), and 6 h (S/G2 phase) before harvesting. Cells were harvested at the end of the 72-h culture period with 1-h colcemid treatment to accumulate mitosis, and further prepared by standard cytogenetic technique. BIDA induced chromatic type breakages and chromatid exchanges at both 1 h and 6 h. The mean number of breakages per cell was 0, 0.1, 1.0, and 1.7 after treatment with 0.1, 0.25, and 0.75 kg/ml, respectively. At 1 kg/ml, BIDA severely inhibited cell progression and very few mitoses were observed. At 6 h the mean number of breakages per cell was 0.3 at 0.25 fig/ml and 1.2 at 0.5 kg/ml. Very few cells entered mitosis at 0.75 and 1 kg/ml. To study the effect of BIDA on cells in G0 and G1, BIDA (0.75 kg/ml) was added for 1 h to the cultures at the beginning of culture (G0), or 24 h after (G1) culture initiation. Afterward, cells were washed and reincubated in the conditioned medium for 71 or 47 h. No chromosomal aberrations were seen in these experiments. The number of chromatid breaks was minimal (0.1 to 0.4/cell). Our study suggests that BID A induces chromatid type aberrations during G2 and S phases. The absence of chromosome type aberrations in cells treated during G0 and G1 suggests that either BID A has no effect on these cells or that damaged cells fail to progress through S and G2 to reach mitosis.",
author = "Niramol Savaraj and Jan Liang and Katherine Lu and Feun, {Lynn G} and Hsu, {T. C.}",
year = "1989",
month = "1",
day = "1",
doi = "10.3109/07357908909038278",
language = "English",
volume = "7",
pages = "117--121",
journal = "Cancer Investigation",
issn = "0735-7907",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Genotoxicity of [1h]benz [de] isoquinoline-1, 3[2h]dione, 5 amino-2-, [2-(dimethylamino) ethyl] (BIDA) in human lymphocytes

AU - Savaraj, Niramol

AU - Liang, Jan

AU - Lu, Katherine

AU - Feun, Lynn G

AU - Hsu, T. C.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - We have investigated the genotoxicity of BIDA in cultured human lymphocytes. Lymphocytes were cultured and stimulated with phytohemagglutinin (PHA) for 72 h. Doses of 0.1, 0.25, 0.5, 0.75, and 1 fig/ml of BIDA were added to the culture at 1 h (G2 phase), and 6 h (S/G2 phase) before harvesting. Cells were harvested at the end of the 72-h culture period with 1-h colcemid treatment to accumulate mitosis, and further prepared by standard cytogenetic technique. BIDA induced chromatic type breakages and chromatid exchanges at both 1 h and 6 h. The mean number of breakages per cell was 0, 0.1, 1.0, and 1.7 after treatment with 0.1, 0.25, and 0.75 kg/ml, respectively. At 1 kg/ml, BIDA severely inhibited cell progression and very few mitoses were observed. At 6 h the mean number of breakages per cell was 0.3 at 0.25 fig/ml and 1.2 at 0.5 kg/ml. Very few cells entered mitosis at 0.75 and 1 kg/ml. To study the effect of BIDA on cells in G0 and G1, BIDA (0.75 kg/ml) was added for 1 h to the cultures at the beginning of culture (G0), or 24 h after (G1) culture initiation. Afterward, cells were washed and reincubated in the conditioned medium for 71 or 47 h. No chromosomal aberrations were seen in these experiments. The number of chromatid breaks was minimal (0.1 to 0.4/cell). Our study suggests that BID A induces chromatid type aberrations during G2 and S phases. The absence of chromosome type aberrations in cells treated during G0 and G1 suggests that either BID A has no effect on these cells or that damaged cells fail to progress through S and G2 to reach mitosis.

AB - We have investigated the genotoxicity of BIDA in cultured human lymphocytes. Lymphocytes were cultured and stimulated with phytohemagglutinin (PHA) for 72 h. Doses of 0.1, 0.25, 0.5, 0.75, and 1 fig/ml of BIDA were added to the culture at 1 h (G2 phase), and 6 h (S/G2 phase) before harvesting. Cells were harvested at the end of the 72-h culture period with 1-h colcemid treatment to accumulate mitosis, and further prepared by standard cytogenetic technique. BIDA induced chromatic type breakages and chromatid exchanges at both 1 h and 6 h. The mean number of breakages per cell was 0, 0.1, 1.0, and 1.7 after treatment with 0.1, 0.25, and 0.75 kg/ml, respectively. At 1 kg/ml, BIDA severely inhibited cell progression and very few mitoses were observed. At 6 h the mean number of breakages per cell was 0.3 at 0.25 fig/ml and 1.2 at 0.5 kg/ml. Very few cells entered mitosis at 0.75 and 1 kg/ml. To study the effect of BIDA on cells in G0 and G1, BIDA (0.75 kg/ml) was added for 1 h to the cultures at the beginning of culture (G0), or 24 h after (G1) culture initiation. Afterward, cells were washed and reincubated in the conditioned medium for 71 or 47 h. No chromosomal aberrations were seen in these experiments. The number of chromatid breaks was minimal (0.1 to 0.4/cell). Our study suggests that BID A induces chromatid type aberrations during G2 and S phases. The absence of chromosome type aberrations in cells treated during G0 and G1 suggests that either BID A has no effect on these cells or that damaged cells fail to progress through S and G2 to reach mitosis.

UR - http://www.scopus.com/inward/record.url?scp=0024474022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024474022&partnerID=8YFLogxK

U2 - 10.3109/07357908909038278

DO - 10.3109/07357908909038278

M3 - Article

VL - 7

SP - 117

EP - 121

JO - Cancer Investigation

JF - Cancer Investigation

SN - 0735-7907

IS - 2

ER -