Genomic structure of the human PLZF gene

E. M. Van Schothorst, D. E M Prins, B. E. Baysal, M. Beekman, J. D. Licht, S. Waxman, Arthur Z Zelent, C. J. Cornelisse, G. J B Van Ommen, C. W. Richard, P. Devilee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The human PLZF (promyelocytic leukaemia zinc finger) gene encodes a Kruppel-like zinc finger protein, which was identified via the reciprocal translocation t(11;17) (q23;q21) fusing it to the retinoic acid receptor alpha (RARa) gene in promyelocytic leukaemia. To determine its complete genomic organisation, we constructed a cosmid-map fully containing the hPLZF gene. The gene has seven exons, including a novel 5' untranslated exon, varying in size from 87 to 1358 bp and spans at least 120 kb. Flanking intronic sequences were identified and all splice acceptor and donor sites conformed to the gt/ag rule. Five polymorphic markers could be fine located in its vicinity. These data will facilitate mutation analysis of hPLZF in t(11;17) leukaemia cases, as well as assist mapping and loss-of-heterozygosity analysis. Here we have tested hPLZF as a possible candidate for the PGL1 locus involved in hereditary head and neck paragangliomas. However, mutation analysis revealed no aberration in 12 paraganglioma patients from different families. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)21-24
Number of pages4
JournalGene
Volume236
Issue number1
DOIs
StatePublished - Aug 5 1999
Externally publishedYes

Fingerprint

Zinc Fingers
Leukemia
Paraganglioma
RNA Splice Sites
Genes
Exons
Cosmids
Mutation
Loss of Heterozygosity
Neck
Head
Proteins

Keywords

  • Chromosome 11
  • Gene organisation
  • Physical map
  • Polymorphic markers

ASJC Scopus subject areas

  • Genetics

Cite this

Van Schothorst, E. M., Prins, D. E. M., Baysal, B. E., Beekman, M., Licht, J. D., Waxman, S., ... Devilee, P. (1999). Genomic structure of the human PLZF gene. Gene, 236(1), 21-24. https://doi.org/10.1016/S0378-1119(99)00277-2

Genomic structure of the human PLZF gene. / Van Schothorst, E. M.; Prins, D. E M; Baysal, B. E.; Beekman, M.; Licht, J. D.; Waxman, S.; Zelent, Arthur Z; Cornelisse, C. J.; Van Ommen, G. J B; Richard, C. W.; Devilee, P.

In: Gene, Vol. 236, No. 1, 05.08.1999, p. 21-24.

Research output: Contribution to journalArticle

Van Schothorst, EM, Prins, DEM, Baysal, BE, Beekman, M, Licht, JD, Waxman, S, Zelent, AZ, Cornelisse, CJ, Van Ommen, GJB, Richard, CW & Devilee, P 1999, 'Genomic structure of the human PLZF gene', Gene, vol. 236, no. 1, pp. 21-24. https://doi.org/10.1016/S0378-1119(99)00277-2
Van Schothorst EM, Prins DEM, Baysal BE, Beekman M, Licht JD, Waxman S et al. Genomic structure of the human PLZF gene. Gene. 1999 Aug 5;236(1):21-24. https://doi.org/10.1016/S0378-1119(99)00277-2
Van Schothorst, E. M. ; Prins, D. E M ; Baysal, B. E. ; Beekman, M. ; Licht, J. D. ; Waxman, S. ; Zelent, Arthur Z ; Cornelisse, C. J. ; Van Ommen, G. J B ; Richard, C. W. ; Devilee, P. / Genomic structure of the human PLZF gene. In: Gene. 1999 ; Vol. 236, No. 1. pp. 21-24.
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