Abstract
Embryonic stem cells, mesenchymal stem cells and induced pluripotent stem cells expanded in vitro exhibit genomic instability. Commonly reported abnormalities include aneuploidy, deletions and duplications (including regions also amplified in cancer). Genomic instability confers an increased risk of malignant transformation that may impact the safety of cultured stem cell transplantation. Possible mechanisms responsible for this genomic instability include DNA repair mechanism abnormalities, telomere crisis, mitotic spindle abnormalities and inappropriate induction of meiotic pathways. Prior to widespread use of these cells in regenerative medicine, it will be critical to gain an understanding of the mechanisms responsible for genomic instability to develop strategies to prevent the accrual of chromosomal defects during expansion in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 653-662 |
| Number of pages | 10 |
| Journal | Regenerative Medicine |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 1 2011 |
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Keywords
- aneuploidy
- B-Myb
- chromosomal instability
- embryonic stem cell
- induced pluripotent stem cell
- meiomitosis
- mesenchymal stem cell
- stem cell
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology
Cite this
Genomic instability in cultured stem cells : Associated risks and underlying mechanisms. / Ross, Andrew L.; Leder, Daniel E.; Weiss, Jonathan; Izakovic, Jan; Grichnik, James M.
In: Regenerative Medicine, Vol. 6, No. 5, 01.09.2011, p. 653-662.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic instability in cultured stem cells
T2 - Associated risks and underlying mechanisms
AU - Ross, Andrew L.
AU - Leder, Daniel E.
AU - Weiss, Jonathan
AU - Izakovic, Jan
AU - Grichnik, James M
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Embryonic stem cells, mesenchymal stem cells and induced pluripotent stem cells expanded in vitro exhibit genomic instability. Commonly reported abnormalities include aneuploidy, deletions and duplications (including regions also amplified in cancer). Genomic instability confers an increased risk of malignant transformation that may impact the safety of cultured stem cell transplantation. Possible mechanisms responsible for this genomic instability include DNA repair mechanism abnormalities, telomere crisis, mitotic spindle abnormalities and inappropriate induction of meiotic pathways. Prior to widespread use of these cells in regenerative medicine, it will be critical to gain an understanding of the mechanisms responsible for genomic instability to develop strategies to prevent the accrual of chromosomal defects during expansion in vitro.
AB - Embryonic stem cells, mesenchymal stem cells and induced pluripotent stem cells expanded in vitro exhibit genomic instability. Commonly reported abnormalities include aneuploidy, deletions and duplications (including regions also amplified in cancer). Genomic instability confers an increased risk of malignant transformation that may impact the safety of cultured stem cell transplantation. Possible mechanisms responsible for this genomic instability include DNA repair mechanism abnormalities, telomere crisis, mitotic spindle abnormalities and inappropriate induction of meiotic pathways. Prior to widespread use of these cells in regenerative medicine, it will be critical to gain an understanding of the mechanisms responsible for genomic instability to develop strategies to prevent the accrual of chromosomal defects during expansion in vitro.
KW - aneuploidy
KW - B-Myb
KW - chromosomal instability
KW - embryonic stem cell
KW - induced pluripotent stem cell
KW - meiomitosis
KW - mesenchymal stem cell
KW - stem cell
UR - http://www.scopus.com/inward/record.url?scp=80052903851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052903851&partnerID=8YFLogxK
U2 - 10.2217/rme.11.44
DO - 10.2217/rme.11.44
M3 - Article
C2 - 21916599
AN - SCOPUS:80052903851
VL - 6
SP - 653
EP - 662
JO - Regenerative Medicine
JF - Regenerative Medicine
SN - 1746-0751
IS - 5
ER -