Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers

Jeffery P. Demuth, Douglas W. Drury, Morgan L. Peters, James Van Dyken, Nicholas K. Priest, Michael J. Wade

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies.

Original languageEnglish (US)
Pages (from-to)1189-1195
Number of pages7
JournalMolecular Ecology Notes
Volume7
Issue number6
DOIs
StatePublished - Nov 2007
Externally publishedYes

Fingerprint

Tribolium
Tribolium castaneum
Microsatellite Repeats
genome
Genes
Genome
microsatellite repeats
heterozygosity
polymorphism
Polymorphism
DNA
genetic polymorphism
Chemical analysis
Population
Surveys and Questionnaires
marker
sampling
distribution

Keywords

  • Microsatellites
  • Tribolium castaneum
  • Whole genome

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers. / Demuth, Jeffery P.; Drury, Douglas W.; Peters, Morgan L.; Van Dyken, James; Priest, Nicholas K.; Wade, Michael J.

In: Molecular Ecology Notes, Vol. 7, No. 6, 11.2007, p. 1189-1195.

Research output: Contribution to journalArticle

Demuth, Jeffery P. ; Drury, Douglas W. ; Peters, Morgan L. ; Van Dyken, James ; Priest, Nicholas K. ; Wade, Michael J. / Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers. In: Molecular Ecology Notes. 2007 ; Vol. 7, No. 6. pp. 1189-1195.
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