Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers

Jeffery P. Demuth, Douglas W. Drury, Morgan L. Peters, J. David Van Dyken, Nicholas K. Priest, Michael J. Wade

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies.

Original languageEnglish (US)
Pages (from-to)1189-1195
Number of pages7
JournalMolecular Ecology Notes
Volume7
Issue number6
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

Keywords

  • Microsatellites
  • Tribolium castaneum
  • Whole genome

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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