Genome-wide DNA methylation levels and altered cortisol stress reactivity following childhood trauma in humans

Lotte C. Houtepen, Christiaan H. Vinkers, Tania Carrillo-Roa, Marieke Hiemstra, Pol A. Van Lier, Wim Meeus, Susan Branje, Christine M. Heim, Charles Nemeroff, Jonathan Mill, Leonard C. Schalkwyk, Menno P. Creyghton, René S. Kahn, Marian Joëls, Elisabeth B. Binder, Marco P M Boks

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

DNA methylation likely plays a role in the regulation of human stress reactivity. Here we show that in a genome-wide analysis of blood DNA methylation in 85 healthy individuals, a locus in the Kit ligand gene (KITLG; cg27512205) showed the strongest association with cortisol stress reactivity (P=5.8 × 10-6). Replication was obtained in two independent samples using either blood (N=45, P=0.001) or buccal cells (N=255, P=0.004). KITLG methylation strongly mediates the relationship between childhood trauma and cortisol stress reactivity in the discovery sample (32% mediation). Its genomic location, a CpG island shore within an H3K27ac enhancer mark, and the correlation between methylation in the blood and prefrontal cortex provide further evidence that KITLG methylation is functionally relevant for the programming of stress reactivity in the human brain. Our results extend preclinical evidence for epigenetic regulation of stress reactivity to humans and provide leads to enhance our understanding of the neurobiological pathways underlying stress vulnerability.

Original languageEnglish (US)
Article number10967
JournalNature Communications
Volume7
DOIs
StatePublished - Mar 21 2016

Fingerprint

methylation
genome
DNA Methylation
Methylation
Hydrocortisone
deoxyribonucleic acid
reactivity
Genes
Genome
Wounds and Injuries
blood
CpG Islands
Stem Cell Factor
Blood
Cheek
Prefrontal Cortex
Epigenomics
mediation
kits
vulnerability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Houtepen, L. C., Vinkers, C. H., Carrillo-Roa, T., Hiemstra, M., Van Lier, P. A., Meeus, W., ... Boks, M. P. M. (2016). Genome-wide DNA methylation levels and altered cortisol stress reactivity following childhood trauma in humans. Nature Communications, 7, [10967]. https://doi.org/10.1038/ncomms10967

Genome-wide DNA methylation levels and altered cortisol stress reactivity following childhood trauma in humans. / Houtepen, Lotte C.; Vinkers, Christiaan H.; Carrillo-Roa, Tania; Hiemstra, Marieke; Van Lier, Pol A.; Meeus, Wim; Branje, Susan; Heim, Christine M.; Nemeroff, Charles; Mill, Jonathan; Schalkwyk, Leonard C.; Creyghton, Menno P.; Kahn, René S.; Joëls, Marian; Binder, Elisabeth B.; Boks, Marco P M.

In: Nature Communications, Vol. 7, 10967, 21.03.2016.

Research output: Contribution to journalArticle

Houtepen, LC, Vinkers, CH, Carrillo-Roa, T, Hiemstra, M, Van Lier, PA, Meeus, W, Branje, S, Heim, CM, Nemeroff, C, Mill, J, Schalkwyk, LC, Creyghton, MP, Kahn, RS, Joëls, M, Binder, EB & Boks, MPM 2016, 'Genome-wide DNA methylation levels and altered cortisol stress reactivity following childhood trauma in humans', Nature Communications, vol. 7, 10967. https://doi.org/10.1038/ncomms10967
Houtepen, Lotte C. ; Vinkers, Christiaan H. ; Carrillo-Roa, Tania ; Hiemstra, Marieke ; Van Lier, Pol A. ; Meeus, Wim ; Branje, Susan ; Heim, Christine M. ; Nemeroff, Charles ; Mill, Jonathan ; Schalkwyk, Leonard C. ; Creyghton, Menno P. ; Kahn, René S. ; Joëls, Marian ; Binder, Elisabeth B. ; Boks, Marco P M. / Genome-wide DNA methylation levels and altered cortisol stress reactivity following childhood trauma in humans. In: Nature Communications. 2016 ; Vol. 7.
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