Genome-Wide Analysis of Central Corneal Thickness in Primary Open-Angle Glaucoma Cases in the NEIGHBOR and GLAUGEN Consortia

Megan Ulmer, Jun Li, Brian L. Yaspan, Ayse Bilge Ozel, Julia E. Richards, Sayoko E. Moroi, Felicia Hawthorne, Donald L. Budenz, David S. Friedman, Douglas Gaasterland, Jonathan Haines, Jae H. Kang, Richard Lee, Paul Lichter, Yutao Liu, Louis R. Pasquale, Margaret Pericak-Vance, Anthony Realini, Joel S. Schuman, Kuldev SinghDouglas Vollrath, Robert Weinreb, Gadi Wollstein, Donald J. Zack, Kang Zhang, Terri Young, R. Rand Allingham, Janey L. Wiggs, Allison Ashley-Koch, Michael A. Hauser

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Purpose. To investigate the effects of central corneal thickness (CCT)-associated variants on primary open-angle glaucoma (POAG) risk using single nucleotide polymorphisms (SNP) data from the Glaucoma Genes and Environment (GLAUGEN) and National Eye Institute (NEI) Glaucoma Human Genetics Collaboration (NEIGHBOR) consortia. Methods. A replication analysis of previously reported CCT SNPs was performed in a CCT dataset (n = 1117) and these SNPs were then tested for association with POAG using a larger POAG dataset (n = 6470). Then a CCT genome-wide association study (GWAS) was performed. Top SNPs from this analysis were selected and tested for association with POAG. cDNA libraries from fetal and adult brain and ocular tissue samples were generated and used for candidate gene expression analysis. Results. Association with one of 20 previously published CCT SNPs was replicated: rs12447690, near the ZNF469 gene (P = 0.001; β = -5.08 μm/allele). None of these SNPs were significantly associated with POAG. In the CCT GWAS, no SNPs reached genome-wide significance. After testing 50 candidate SNPs for association with POAG, one SNP was identified, rs7481514 within the neurotrimin (NTM) gene, that was significantly associated with POAG in a low-tension subset (P = 0.00099; Odds Ratio [OR] = 1.28). Additionally, SNPs in the CNTNAP4 gene showed suggestive association with POAG (top SNP = rs1428758; P = 0.018; OR = 0.84). NTM and CNTNAP4 were shown to be expressed in ocular tissues. Conclusions. The results suggest previously reported CCT loci are not significantly associated with POAG susceptibility. By performing a quantitative analysis of CCT and a subsequent analysis of POAG, SNPs in two cell adhesion molecules, NTM and CNTNAP4, were identified and may increase POAG susceptibility in a subset of cases.

Original languageEnglish (US)
Pages (from-to)4468-4474
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number8
DOIs
StatePublished - Jul 1 2012

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ulmer, M., Li, J., Yaspan, B. L., Ozel, A. B., Richards, J. E., Moroi, S. E., Hawthorne, F., Budenz, D. L., Friedman, D. S., Gaasterland, D., Haines, J., Kang, J. H., Lee, R., Lichter, P., Liu, Y., Pasquale, L. R., Pericak-Vance, M., Realini, A., Schuman, J. S., ... Hauser, M. A. (2012). Genome-Wide Analysis of Central Corneal Thickness in Primary Open-Angle Glaucoma Cases in the NEIGHBOR and GLAUGEN Consortia. Investigative Ophthalmology and Visual Science, 53(8), 4468-4474. https://doi.org/10.1167/iovs.12-9784