Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites

Jay Z. Parrish, Michael D. Kim, Yeh Jan Lily, Nung Jan Yuh

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis of transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 transcriptional regulators and identified 78 genes involved in patterning the stereotyped dendritic arbors of class I da neurons in Drosophila. Most of these transcriptional regulators affect dendrite morphology without altering the number of class I dendrite arborization (da) neurons and fall primarily into three groups. Group A genes control both primary dendrite extension and lateral branching, hence the overall dendritic field. Nineteen genes within group A act to increase arborization, whereas 20 other genes restrict dendritic coverage. Group B genes appear to balance dendritic outgrowth and branching. Nineteen group B genes function to promote branching rather than outgrowth, and two others have the opposite effects. Finally, 10 group C genes are critical for the routing of the dendritic arbors of individual class I da neurons. Thus, multiple genetic programs operate to calibrate dendritic coverage, to coordinate the elaboration of primary versus secondary branches, and to lay out these dendritic branches in the proper orientation.

Original languageEnglish
Pages (from-to)820-835
Number of pages16
JournalGenes and Development
Volume20
Issue number7
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Fingerprint

Sensory Receptor Cells
Dendrites
Morphogenesis
Drosophila
Neuronal Plasticity
Transcription Factors
Genome
Genes
Neurons
vif Genes
RNA Interference

Keywords

  • Dendrite
  • Drosophila
  • Neuron
  • RNAi
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. / Parrish, Jay Z.; Kim, Michael D.; Lily, Yeh Jan; Yuh, Nung Jan.

In: Genes and Development, Vol. 20, No. 7, 01.04.2006, p. 820-835.

Research output: Contribution to journalArticle

Parrish, Jay Z. ; Kim, Michael D. ; Lily, Yeh Jan ; Yuh, Nung Jan. / Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. In: Genes and Development. 2006 ; Vol. 20, No. 7. pp. 820-835.
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