Genome sequence of Yersinia pestis KIM

Wen Deng, Valerie Burland, Guy Plunkett, Adam Boutin, George F. Mayhew, Paul Liss, Nicole T. Perna, Debra J. Rose, Bob Mau, Shiguo Zhou, David C. Schwartz, Jaqueline D. Fetherston, Luther E. Lindler, Robert R. Brubaker, Gregory V. Plano, Susan C. Straley, Kathleen A. McDonough, Matthew L. Nilles, Jyl S. Matson, Frederick R. BlattnerRobert D. Perry

Research output: Contribution to journalArticle

426 Scopus citations

Abstract

We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes encoding DNA replication proteins are similar to those of Escherichia coli K-12. The KIM genome sequence was compared with that of Y. pestis CO92, biovar Orientalis, revealing homologous sequences but a remarkable amount of genome rearrangement for strains so closely related. The differences appear to result from multiple inversions of genome segments at insertion sequences, in a manner consistent with present knowledge of replication and recombination. There are few differences attributable to horizontal transfer. The KIM and E. coli K-12 genome proteins were also compared, exposing surprising amounts of locally colinear "backbone," or synteny, that is not discernible at the nucleotide level. Nearly 54% of KIM ORFs are significantly similar to K-12 proteins, with conserved housekeeping functions. However, a number of E. coli pathways and transport systems and at least one global regulator were not found, reflecting differences in lifestyle between them. In KIM-specific islands, new genes encode candidate pathogenicity proteins, including iron transport systems, putative adhesins, toxins, and fimbriae.

Original languageEnglish (US)
Pages (from-to)4601-4611
Number of pages11
JournalJournal of bacteriology
Volume184
Issue number16
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Genome sequence of Yersinia pestis KIM'. Together they form a unique fingerprint.

  • Cite this

    Deng, W., Burland, V., Plunkett, G., Boutin, A., Mayhew, G. F., Liss, P., Perna, N. T., Rose, D. J., Mau, B., Zhou, S., Schwartz, D. C., Fetherston, J. D., Lindler, L. E., Brubaker, R. R., Plano, G. V., Straley, S. C., McDonough, K. A., Nilles, M. L., Matson, J. S., ... Perry, R. D. (2002). Genome sequence of Yersinia pestis KIM. Journal of bacteriology, 184(16), 4601-4611. https://doi.org/10.1128/JB.184.16.4601-4611.2002