Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen

Dragana Ajdic, William M. McShan, Robert E. McLaughlin, Gorana Savić, Jin Chang, Matthew B. Carson, Charles Primeaux, Runying Tian, Steve Kenton, Honggui Jia, Shaoping Lin, Yudong Qian, Shuling Li, Hua Zhu, Fares Najar, Hongshing Lai, Jim White, Bruce A. Roe, Joseph J. Ferretti

Research output: Contribution to journalArticle

673 Citations (Scopus)

Abstract

Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA159, a serotype c strain, has been completely sequenced and is composed of 2,030,936 base pairs. It contains 1,963 ORFs, 63% of which have been assigned putative functions. The genome analysis provides further insight into how S. mutans has adapted to surviving the oral environment through resource acquisition, defense against host factors, and use of gene products that maintain its niche against microbial competitors. S. mutans metabolizes a wide variety of carbohydrates via nonoxidative pathways, and all of these pathways have been identified, along with the associated transport systems whose genes account for almost 15% of the genome. Virulence genes associated with extracellular adherent glucan production, adhesins, acid tolerance, proteases, and putative hemolysins have been identified. Strain UA159 is naturally competent and contains all of the genes essential for competence and quorum sensing. Mobile genetic elements in the form of IS elements and transposons are prominent in the genome and include a previously uncharacterized conjugative transposon and a composite transposon containing genes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage genomes are present.

Original languageEnglish
Pages (from-to)14434-14439
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number22
DOIs
StatePublished - Oct 29 2002
Externally publishedYes

Fingerprint

Streptococcus mutans
Tooth
Genome
Genes
Interspersed Repetitive Sequences
Bacitracin
Gramicidin
Quorum Sensing
Hemolysin Proteins
DNA Transposable Elements
Glucans
Essential Genes
Dental Caries
Streptococcus
Base Pairing
Bacteriophages
Mental Competency
Open Reading Frames
Virulence
Peptide Hydrolases

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. / Ajdic, Dragana; McShan, William M.; McLaughlin, Robert E.; Savić, Gorana; Chang, Jin; Carson, Matthew B.; Primeaux, Charles; Tian, Runying; Kenton, Steve; Jia, Honggui; Lin, Shaoping; Qian, Yudong; Li, Shuling; Zhu, Hua; Najar, Fares; Lai, Hongshing; White, Jim; Roe, Bruce A.; Ferretti, Joseph J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 22, 29.10.2002, p. 14434-14439.

Research output: Contribution to journalArticle

Ajdic, D, McShan, WM, McLaughlin, RE, Savić, G, Chang, J, Carson, MB, Primeaux, C, Tian, R, Kenton, S, Jia, H, Lin, S, Qian, Y, Li, S, Zhu, H, Najar, F, Lai, H, White, J, Roe, BA & Ferretti, JJ 2002, 'Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 22, pp. 14434-14439. https://doi.org/10.1073/pnas.172501299
Ajdic, Dragana ; McShan, William M. ; McLaughlin, Robert E. ; Savić, Gorana ; Chang, Jin ; Carson, Matthew B. ; Primeaux, Charles ; Tian, Runying ; Kenton, Steve ; Jia, Honggui ; Lin, Shaoping ; Qian, Yudong ; Li, Shuling ; Zhu, Hua ; Najar, Fares ; Lai, Hongshing ; White, Jim ; Roe, Bruce A. ; Ferretti, Joseph J. / Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 22. pp. 14434-14439.
@article{d52ed28b03a8492c98124df87d9c1cbe,
title = "Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen",
abstract = "Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA159, a serotype c strain, has been completely sequenced and is composed of 2,030,936 base pairs. It contains 1,963 ORFs, 63{\%} of which have been assigned putative functions. The genome analysis provides further insight into how S. mutans has adapted to surviving the oral environment through resource acquisition, defense against host factors, and use of gene products that maintain its niche against microbial competitors. S. mutans metabolizes a wide variety of carbohydrates via nonoxidative pathways, and all of these pathways have been identified, along with the associated transport systems whose genes account for almost 15{\%} of the genome. Virulence genes associated with extracellular adherent glucan production, adhesins, acid tolerance, proteases, and putative hemolysins have been identified. Strain UA159 is naturally competent and contains all of the genes essential for competence and quorum sensing. Mobile genetic elements in the form of IS elements and transposons are prominent in the genome and include a previously uncharacterized conjugative transposon and a composite transposon containing genes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage genomes are present.",
author = "Dragana Ajdic and McShan, {William M.} and McLaughlin, {Robert E.} and Gorana Savić and Jin Chang and Carson, {Matthew B.} and Charles Primeaux and Runying Tian and Steve Kenton and Honggui Jia and Shaoping Lin and Yudong Qian and Shuling Li and Hua Zhu and Fares Najar and Hongshing Lai and Jim White and Roe, {Bruce A.} and Ferretti, {Joseph J.}",
year = "2002",
month = "10",
day = "29",
doi = "10.1073/pnas.172501299",
language = "English",
volume = "99",
pages = "14434--14439",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "22",

}

TY - JOUR

T1 - Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen

AU - Ajdic, Dragana

AU - McShan, William M.

AU - McLaughlin, Robert E.

AU - Savić, Gorana

AU - Chang, Jin

AU - Carson, Matthew B.

AU - Primeaux, Charles

AU - Tian, Runying

AU - Kenton, Steve

AU - Jia, Honggui

AU - Lin, Shaoping

AU - Qian, Yudong

AU - Li, Shuling

AU - Zhu, Hua

AU - Najar, Fares

AU - Lai, Hongshing

AU - White, Jim

AU - Roe, Bruce A.

AU - Ferretti, Joseph J.

PY - 2002/10/29

Y1 - 2002/10/29

N2 - Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA159, a serotype c strain, has been completely sequenced and is composed of 2,030,936 base pairs. It contains 1,963 ORFs, 63% of which have been assigned putative functions. The genome analysis provides further insight into how S. mutans has adapted to surviving the oral environment through resource acquisition, defense against host factors, and use of gene products that maintain its niche against microbial competitors. S. mutans metabolizes a wide variety of carbohydrates via nonoxidative pathways, and all of these pathways have been identified, along with the associated transport systems whose genes account for almost 15% of the genome. Virulence genes associated with extracellular adherent glucan production, adhesins, acid tolerance, proteases, and putative hemolysins have been identified. Strain UA159 is naturally competent and contains all of the genes essential for competence and quorum sensing. Mobile genetic elements in the form of IS elements and transposons are prominent in the genome and include a previously uncharacterized conjugative transposon and a composite transposon containing genes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage genomes are present.

AB - Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA159, a serotype c strain, has been completely sequenced and is composed of 2,030,936 base pairs. It contains 1,963 ORFs, 63% of which have been assigned putative functions. The genome analysis provides further insight into how S. mutans has adapted to surviving the oral environment through resource acquisition, defense against host factors, and use of gene products that maintain its niche against microbial competitors. S. mutans metabolizes a wide variety of carbohydrates via nonoxidative pathways, and all of these pathways have been identified, along with the associated transport systems whose genes account for almost 15% of the genome. Virulence genes associated with extracellular adherent glucan production, adhesins, acid tolerance, proteases, and putative hemolysins have been identified. Strain UA159 is naturally competent and contains all of the genes essential for competence and quorum sensing. Mobile genetic elements in the form of IS elements and transposons are prominent in the genome and include a previously uncharacterized conjugative transposon and a composite transposon containing genes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage genomes are present.

UR - http://www.scopus.com/inward/record.url?scp=0037195085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037195085&partnerID=8YFLogxK

U2 - 10.1073/pnas.172501299

DO - 10.1073/pnas.172501299

M3 - Article

C2 - 12397186

AN - SCOPUS:0037195085

VL - 99

SP - 14434

EP - 14439

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 22

ER -