Genetic mapping of a mutation in Escherichia coli leading to a temperature-sensitive RNase D

Richard Zaniewski, Murray P Deutscher

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In order to determine the metabolic role of RNase D in Escherichia coli, we have attempted to isolate strains deficient in this enzyme. One strain containing a temperature-sensitive RNase D was found among a heavily mutagenized stock of strains temperature-sensitive for growth. Genetic mapping of the mutation responsible for the altered RNase D enabled us to define the rnd locus, at 39.5-40.0 min on the E. coli map, which apparently specifies the RNase D structural gene. Using a Tn 10 insertion near the rnd locus, we constructed isogenic strains containing RNase D and RNase II mutations, alone or in combination. Although the original mutant isolate displayed temperature-sensitive growth, no growth phenotype was associated with the rnd mutation in wild type background, possibly because a substantial amount of RNase D remained in cells grown at 45° C. However, elucidation of the map position of the rnd locus should prove useful for the isolation of other mutant strains with lower levels of RNase D.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalMGG Molecular & General Genetics
Volume185
Issue number1
DOIs
StatePublished - Mar 1 1982
Externally publishedYes

Fingerprint

Ribonuclease III
Escherichia coli
Mutation
Temperature
Growth
Phenotype
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Genetic mapping of a mutation in Escherichia coli leading to a temperature-sensitive RNase D. / Zaniewski, Richard; Deutscher, Murray P.

In: MGG Molecular & General Genetics, Vol. 185, No. 1, 01.03.1982, p. 142-147.

Research output: Contribution to journalArticle

Zaniewski, Richard ; Deutscher, Murray P. / Genetic mapping of a mutation in Escherichia coli leading to a temperature-sensitive RNase D. In: MGG Molecular & General Genetics. 1982 ; Vol. 185, No. 1. pp. 142-147.
@article{d554784b8a244c4ab34d9a515e66e590,
title = "Genetic mapping of a mutation in Escherichia coli leading to a temperature-sensitive RNase D",
abstract = "In order to determine the metabolic role of RNase D in Escherichia coli, we have attempted to isolate strains deficient in this enzyme. One strain containing a temperature-sensitive RNase D was found among a heavily mutagenized stock of strains temperature-sensitive for growth. Genetic mapping of the mutation responsible for the altered RNase D enabled us to define the rnd locus, at 39.5-40.0 min on the E. coli map, which apparently specifies the RNase D structural gene. Using a Tn 10 insertion near the rnd locus, we constructed isogenic strains containing RNase D and RNase II mutations, alone or in combination. Although the original mutant isolate displayed temperature-sensitive growth, no growth phenotype was associated with the rnd mutation in wild type background, possibly because a substantial amount of RNase D remained in cells grown at 45° C. However, elucidation of the map position of the rnd locus should prove useful for the isolation of other mutant strains with lower levels of RNase D.",
author = "Richard Zaniewski and Deutscher, {Murray P}",
year = "1982",
month = "3",
day = "1",
doi = "10.1007/BF00333804",
language = "English",
volume = "185",
pages = "142--147",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Genetic mapping of a mutation in Escherichia coli leading to a temperature-sensitive RNase D

AU - Zaniewski, Richard

AU - Deutscher, Murray P

PY - 1982/3/1

Y1 - 1982/3/1

N2 - In order to determine the metabolic role of RNase D in Escherichia coli, we have attempted to isolate strains deficient in this enzyme. One strain containing a temperature-sensitive RNase D was found among a heavily mutagenized stock of strains temperature-sensitive for growth. Genetic mapping of the mutation responsible for the altered RNase D enabled us to define the rnd locus, at 39.5-40.0 min on the E. coli map, which apparently specifies the RNase D structural gene. Using a Tn 10 insertion near the rnd locus, we constructed isogenic strains containing RNase D and RNase II mutations, alone or in combination. Although the original mutant isolate displayed temperature-sensitive growth, no growth phenotype was associated with the rnd mutation in wild type background, possibly because a substantial amount of RNase D remained in cells grown at 45° C. However, elucidation of the map position of the rnd locus should prove useful for the isolation of other mutant strains with lower levels of RNase D.

AB - In order to determine the metabolic role of RNase D in Escherichia coli, we have attempted to isolate strains deficient in this enzyme. One strain containing a temperature-sensitive RNase D was found among a heavily mutagenized stock of strains temperature-sensitive for growth. Genetic mapping of the mutation responsible for the altered RNase D enabled us to define the rnd locus, at 39.5-40.0 min on the E. coli map, which apparently specifies the RNase D structural gene. Using a Tn 10 insertion near the rnd locus, we constructed isogenic strains containing RNase D and RNase II mutations, alone or in combination. Although the original mutant isolate displayed temperature-sensitive growth, no growth phenotype was associated with the rnd mutation in wild type background, possibly because a substantial amount of RNase D remained in cells grown at 45° C. However, elucidation of the map position of the rnd locus should prove useful for the isolation of other mutant strains with lower levels of RNase D.

UR - http://www.scopus.com/inward/record.url?scp=0020036487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020036487&partnerID=8YFLogxK

U2 - 10.1007/BF00333804

DO - 10.1007/BF00333804

M3 - Article

VL - 185

SP - 142

EP - 147

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 1

ER -