Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys

Petr O. Ilyinskii, Muthiah D. Daniel, Christopher J. Horvath, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

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Abstract

Simian virus 40 (SV40) was isolated from the brains of three rhesus monkeys and the kidneys of two other rhesus monkeys with simian immunodeficiency virus-induced immunodeficiency. A striking feature of these five cases was the tissue specificity of the SV40 replication. SV40 was also isolated from the kidney of a Taiwanese rock macaque with immunodeficiency probably caused by type D retrovirus infection. Multiple full-length clones were derived from all six fresh SV40 isolates, and two separate regions of their genomes were sequenced: the origin (ori)-enhancer region and the coding region for the carboxy terminus of T antigen (T-ag). None of the 23 clones analyzed had two 72-bp enhancer elements as are present in the commonly used laboratory strain 776 of SV40; 22 of these 23 clones were identical in their ori-enhancer sequences, and these had only a single 72-bp enhancer element. We found no evidence for differences in ori-enhancer sequences associated with tissue-specific SV40 replication. The T-ag coding sequence that was analyzed was identical in all clones from kidney. However, significant variation was observed in the carboxy-terminal region of T-ag in SV40 isolated from brain tissues. This sequence variation was located in a region previously reported to be responsible for SV40 host range in cultured cell lines. Thus, SV40 appears to be an opportunistic pathogen in the setting of simian immunodeficiency virus-induced immunodeficiency, similarly to JC virus in human immunodeficiency virus-infected humans, the enhancer sequence organization generally attributed to SV40 is not representative of natural SV40 isolates, and sequence variation near the carboxy terminus of T-ag may play a role in tissue-specific replication of SV40.

Original languageEnglish (US)
Pages (from-to)6353-6360
Number of pages8
JournalJournal of Virology
Volume66
Issue number11
StatePublished - Nov 1992
Externally publishedYes

Fingerprint

Simian virus 40
Macaca
Haplorhini
monkeys
genetic techniques and protocols
kidneys
Kidney
brain
Brain
enhancer elements
Viral Tumor Antigens
immunosuppression
Clone Cells
clones
antigens
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Virus Replication
Macaca mulatta
JC polyomavirus

ASJC Scopus subject areas

  • Immunology

Cite this

Ilyinskii, P. O., Daniel, M. D., Horvath, C. J., & Desrosiers, R. C. (1992). Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys. Journal of Virology, 66(11), 6353-6360.

Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys. / Ilyinskii, Petr O.; Daniel, Muthiah D.; Horvath, Christopher J.; Desrosiers, Ronald Charles.

In: Journal of Virology, Vol. 66, No. 11, 11.1992, p. 6353-6360.

Research output: Contribution to journalArticle

Ilyinskii, PO, Daniel, MD, Horvath, CJ & Desrosiers, RC 1992, 'Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys', Journal of Virology, vol. 66, no. 11, pp. 6353-6360.
Ilyinskii, Petr O. ; Daniel, Muthiah D. ; Horvath, Christopher J. ; Desrosiers, Ronald Charles. / Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys. In: Journal of Virology. 1992 ; Vol. 66, No. 11. pp. 6353-6360.
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