Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

Samita Andreansky, J. Stambas, A. Gutierrez, G. Diaz, P. C. Doherty, S. J. Turner, R. J. Webby

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalInternational Congress Series
Volume1263
Issue numberC
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

Fingerprint

T-Lymphocyte Epitopes
Influenza A virus
Cytotoxic T-Lymphocytes
Viruses
Reverse Genetics
Human Influenza
Epitopes
Immunodominant Epitopes
Genetic Models
Infection
Immunity
Vaccination
Mutation
Growth

Keywords

  • CD8
  • Cytotoxic
  • Epitope
  • Influenza

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Andreansky, S., Stambas, J., Gutierrez, A., Diaz, G., Doherty, P. C., Turner, S. J., & Webby, R. J. (2004). Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes. International Congress Series, 1263(C), 141-144. https://doi.org/10.1016/j.ics.2004.01.046

Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes. / Andreansky, Samita; Stambas, J.; Gutierrez, A.; Diaz, G.; Doherty, P. C.; Turner, S. J.; Webby, R. J.

In: International Congress Series, Vol. 1263, No. C, 01.06.2004, p. 141-144.

Research output: Contribution to journalArticle

Andreansky, S, Stambas, J, Gutierrez, A, Diaz, G, Doherty, PC, Turner, SJ & Webby, RJ 2004, 'Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes', International Congress Series, vol. 1263, no. C, pp. 141-144. https://doi.org/10.1016/j.ics.2004.01.046
Andreansky, Samita ; Stambas, J. ; Gutierrez, A. ; Diaz, G. ; Doherty, P. C. ; Turner, S. J. ; Webby, R. J. / Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes. In: International Congress Series. 2004 ; Vol. 1263, No. C. pp. 141-144.
@article{70e485b2c83449dda1135eba2b7f2214,
title = "Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes",
abstract = "The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.",
keywords = "CD8, Cytotoxic, Epitope, Influenza",
author = "Samita Andreansky and J. Stambas and A. Gutierrez and G. Diaz and Doherty, {P. C.} and Turner, {S. J.} and Webby, {R. J.}",
year = "2004",
month = "6",
day = "1",
doi = "10.1016/j.ics.2004.01.046",
language = "English (US)",
volume = "1263",
pages = "141--144",
journal = "Scientific Computing and Instrumentation",
issn = "1078-8956",
publisher = "Springer Wien",
number = "C",

}

TY - JOUR

T1 - Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

AU - Andreansky, Samita

AU - Stambas, J.

AU - Gutierrez, A.

AU - Diaz, G.

AU - Doherty, P. C.

AU - Turner, S. J.

AU - Webby, R. J.

PY - 2004/6/1

Y1 - 2004/6/1

N2 - The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.

AB - The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.

KW - CD8

KW - Cytotoxic

KW - Epitope

KW - Influenza

UR - http://www.scopus.com/inward/record.url?scp=84874757641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874757641&partnerID=8YFLogxK

U2 - 10.1016/j.ics.2004.01.046

DO - 10.1016/j.ics.2004.01.046

M3 - Article

VL - 1263

SP - 141

EP - 144

JO - Scientific Computing and Instrumentation

JF - Scientific Computing and Instrumentation

SN - 1078-8956

IS - C

ER -