Abstract
The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-144 |
| Number of pages | 4 |
| Journal | International Congress Series |
| Volume | 1263 |
| Issue number | C |
| DOIs | |
| State | Published - Jun 1 2004 |
| Externally published | Yes |
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Keywords
- CD8
- Cytotoxic
- Epitope
- Influenza
ASJC Scopus subject areas
- Medicine(all)
Cite this
Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes. / Andreansky, Samita; Stambas, J.; Gutierrez, A.; Diaz, G.; Doherty, P. C.; Turner, S. J.; Webby, R. J.
In: International Congress Series, Vol. 1263, No. C, 01.06.2004, p. 141-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes
AU - Andreansky, Samita
AU - Stambas, J.
AU - Gutierrez, A.
AU - Diaz, G.
AU - Doherty, P. C.
AU - Turner, S. J.
AU - Webby, R. J.
PY - 2004/6/1
Y1 - 2004/6/1
N2 - The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
AB - The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
KW - CD8
KW - Cytotoxic
KW - Epitope
KW - Influenza
UR - http://www.scopus.com/inward/record.url?scp=84874757641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874757641&partnerID=8YFLogxK
U2 - 10.1016/j.ics.2004.01.046
DO - 10.1016/j.ics.2004.01.046
M3 - Article
VL - 1263
SP - 141
EP - 144
JO - Scientific Computing and Instrumentation
JF - Scientific Computing and Instrumentation
SN - 1078-8956
IS - C
ER -