Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors

Bai Liu, Eckhard R. Podack, James P. Allison, Thomas Malek

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study investigated the generation of primary tumor-specific CTL activity in vitro to several mouse tumors. We report that the development of optimal primary tumor-specific CTL to the P815 mastocytoma, the EL4 thymoma, and the Lewis lung carcinoma is dependent on tumor Ags, on enhancement of T cell costimulation by B7.1, and on exogenous T helper activity in the form of IL-2 and IL-4. A relatively low concentration of IL-2 and IL-4 was required to limit the induction of lymphokine-activated killer cells. In the case of P815, the CTL were directed toward molecularly defined tumor rejection Ags. These primary cultures yielded long term T cell lines that were heterogeneous in fine tumor Ag specificity and in cytokine production.

Original languageEnglish
Pages (from-to)1117-1125
Number of pages9
JournalJournal of Immunology
Volume156
Issue number3
StatePublished - Feb 1 1996

Fingerprint

Neoplasms
Interleukin-4
Interleukin-2
Mastocytoma
Lewis Lung Carcinoma
T-Lymphocytes
Lymphokine-Activated Killer Cells
Thymoma
In Vitro Techniques
Cytokines
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors. / Liu, Bai; Podack, Eckhard R.; Allison, James P.; Malek, Thomas.

In: Journal of Immunology, Vol. 156, No. 3, 01.02.1996, p. 1117-1125.

Research output: Contribution to journalArticle

Liu, Bai ; Podack, Eckhard R. ; Allison, James P. ; Malek, Thomas. / Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors. In: Journal of Immunology. 1996 ; Vol. 156, No. 3. pp. 1117-1125.
@article{ec42de14709145dabbcac1b322055715,
title = "Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors",
abstract = "This study investigated the generation of primary tumor-specific CTL activity in vitro to several mouse tumors. We report that the development of optimal primary tumor-specific CTL to the P815 mastocytoma, the EL4 thymoma, and the Lewis lung carcinoma is dependent on tumor Ags, on enhancement of T cell costimulation by B7.1, and on exogenous T helper activity in the form of IL-2 and IL-4. A relatively low concentration of IL-2 and IL-4 was required to limit the induction of lymphokine-activated killer cells. In the case of P815, the CTL were directed toward molecularly defined tumor rejection Ags. These primary cultures yielded long term T cell lines that were heterogeneous in fine tumor Ag specificity and in cytokine production.",
author = "Bai Liu and Podack, {Eckhard R.} and Allison, {James P.} and Thomas Malek",
year = "1996",
month = "2",
day = "1",
language = "English",
volume = "156",
pages = "1117--1125",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors

AU - Liu, Bai

AU - Podack, Eckhard R.

AU - Allison, James P.

AU - Malek, Thomas

PY - 1996/2/1

Y1 - 1996/2/1

N2 - This study investigated the generation of primary tumor-specific CTL activity in vitro to several mouse tumors. We report that the development of optimal primary tumor-specific CTL to the P815 mastocytoma, the EL4 thymoma, and the Lewis lung carcinoma is dependent on tumor Ags, on enhancement of T cell costimulation by B7.1, and on exogenous T helper activity in the form of IL-2 and IL-4. A relatively low concentration of IL-2 and IL-4 was required to limit the induction of lymphokine-activated killer cells. In the case of P815, the CTL were directed toward molecularly defined tumor rejection Ags. These primary cultures yielded long term T cell lines that were heterogeneous in fine tumor Ag specificity and in cytokine production.

AB - This study investigated the generation of primary tumor-specific CTL activity in vitro to several mouse tumors. We report that the development of optimal primary tumor-specific CTL to the P815 mastocytoma, the EL4 thymoma, and the Lewis lung carcinoma is dependent on tumor Ags, on enhancement of T cell costimulation by B7.1, and on exogenous T helper activity in the form of IL-2 and IL-4. A relatively low concentration of IL-2 and IL-4 was required to limit the induction of lymphokine-activated killer cells. In the case of P815, the CTL were directed toward molecularly defined tumor rejection Ags. These primary cultures yielded long term T cell lines that were heterogeneous in fine tumor Ag specificity and in cytokine production.

UR - http://www.scopus.com/inward/record.url?scp=0030070974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030070974&partnerID=8YFLogxK

M3 - Article

VL - 156

SP - 1117

EP - 1125

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -