Generation of multiple monoclonal antibodies for diagnostic use from a single hybridoma fusion

Shinobu Matsuo, Neal S. Penneys, Jocelyn Ziegels Weissman, Mehrdad Nadji

Research output: Contribution to journalArticle

Abstract

BALB/c mice were exposed simultaneously to three nonrelated immunogens, myelin basic protein, uridyl-glactosyl tansferase, and tissue obtained from a formalin-fixed, paraffin-embedded block containing a pilomatrixoma. Standard hybridoma techniques were used and antibody generation assayed using an unlabelled antibody biotin-abidin method with section of human cerebellum, liver, and pilomatrixoma as the substrates. Using the above assay, clones were selected that secreted anibodies with selective specificities for each of the immunogens. By ELISA assay, the monoclonal antibodies reacting with cerebellum also reacted to the myelin basic protein preparation used as immunogen and the monoclonal antibody reacting to hepatocytes bound to the preparation of uridyl-galactosyl transferase used as immunogen. Our data suggests that the generation of monoclonal antibodies with a variety of diagnostic applications can be obtained from a single fusion following immunization with multiple nonrelated antigens, requiring considerably less laboratory cost and effort than would be required to obtain similar monoclonal antibodies in separate fusions.

Original languageEnglish (US)
Pages (from-to)137-139
Number of pages3
JournalActa Haematologica
Volume79
Issue number3
DOIs
StatePublished - Jan 1 1988

Keywords

  • Hybridoma
  • Monoclonal antibodies
  • Multiple antigens

ASJC Scopus subject areas

  • Hematology

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