Abstract
BALB/c mice were exposed simultaneously to three nonrelated immunogens, myelin basic protein, uridyl-glactosyl tansferase, and tissue obtained from a formalin-fixed, paraffin-embedded block containing a pilomatrixoma. Standard hybridoma techniques were used and antibody generation assayed using an unlabelled antibody biotin-abidin method with section of human cerebellum, liver, and pilomatrixoma as the substrates. Using the above assay, clones were selected that secreted anibodies with selective specificities for each of the immunogens. By ELISA assay, the monoclonal antibodies reacting with cerebellum also reacted to the myelin basic protein preparation used as immunogen and the monoclonal antibody reacting to hepatocytes bound to the preparation of uridyl-galactosyl transferase used as immunogen. Our data suggests that the generation of monoclonal antibodies with a variety of diagnostic applications can be obtained from a single fusion following immunization with multiple nonrelated antigens, requiring considerably less laboratory cost and effort than would be required to obtain similar monoclonal antibodies in separate fusions.
| Original language | English |
|---|---|
| Pages (from-to) | 137-139 |
| Number of pages | 3 |
| Journal | Acta Haematologica |
| Volume | 79 |
| Issue number | 3 |
| State | Published - Jan 1 1988 |
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ASJC Scopus subject areas
- Hematology
Cite this
Generation of multiple monoclonal antibodies for diagnostic use from a single hybridoma fusion. / Matsuo, S.; Penneys, N. S.; Ziegels-Weissman, J.; Nadji, Mehrdad.
In: Acta Haematologica, Vol. 79, No. 3, 01.01.1988, p. 137-139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of multiple monoclonal antibodies for diagnostic use from a single hybridoma fusion
AU - Matsuo, S.
AU - Penneys, N. S.
AU - Ziegels-Weissman, J.
AU - Nadji, Mehrdad
PY - 1988/1/1
Y1 - 1988/1/1
N2 - BALB/c mice were exposed simultaneously to three nonrelated immunogens, myelin basic protein, uridyl-glactosyl tansferase, and tissue obtained from a formalin-fixed, paraffin-embedded block containing a pilomatrixoma. Standard hybridoma techniques were used and antibody generation assayed using an unlabelled antibody biotin-abidin method with section of human cerebellum, liver, and pilomatrixoma as the substrates. Using the above assay, clones were selected that secreted anibodies with selective specificities for each of the immunogens. By ELISA assay, the monoclonal antibodies reacting with cerebellum also reacted to the myelin basic protein preparation used as immunogen and the monoclonal antibody reacting to hepatocytes bound to the preparation of uridyl-galactosyl transferase used as immunogen. Our data suggests that the generation of monoclonal antibodies with a variety of diagnostic applications can be obtained from a single fusion following immunization with multiple nonrelated antigens, requiring considerably less laboratory cost and effort than would be required to obtain similar monoclonal antibodies in separate fusions.
AB - BALB/c mice were exposed simultaneously to three nonrelated immunogens, myelin basic protein, uridyl-glactosyl tansferase, and tissue obtained from a formalin-fixed, paraffin-embedded block containing a pilomatrixoma. Standard hybridoma techniques were used and antibody generation assayed using an unlabelled antibody biotin-abidin method with section of human cerebellum, liver, and pilomatrixoma as the substrates. Using the above assay, clones were selected that secreted anibodies with selective specificities for each of the immunogens. By ELISA assay, the monoclonal antibodies reacting with cerebellum also reacted to the myelin basic protein preparation used as immunogen and the monoclonal antibody reacting to hepatocytes bound to the preparation of uridyl-galactosyl transferase used as immunogen. Our data suggests that the generation of monoclonal antibodies with a variety of diagnostic applications can be obtained from a single fusion following immunization with multiple nonrelated antigens, requiring considerably less laboratory cost and effort than would be required to obtain similar monoclonal antibodies in separate fusions.
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M3 - Article
C2 - 2451388
AN - SCOPUS:0023896569
VL - 79
SP - 137
EP - 139
JO - Acta Haematologica
JF - Acta Haematologica
SN - 0001-5792
IS - 3
ER -