Generation of mice with a conditional Lbh null allele

Linsey E. Lindley, Karoline J. Briegel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Limb bud and heart (LBH) is a developmentally expressed, tissue-specific transcription cofactor in vertebrates that acts in the WNT signaling pathway, a genetic program critical for embryogenesis and adult tissue homeostasis. Aberrant gain-of-function of LBH is implicated in both human congenital disease and cancer. The normal physiological function of LBH has remained elusive owing to a lack of genetic loss-of-function models. Here, we have generated mice with a conditional null allele of Lbh by flanking exon 2 with loxP sites (Lbhflox). Homozygous Lbhflox and LbhloxP mice, in which the Neo cassette was removed through FLPe-mediated recombination, were viable and fertile, indicating that these conditional Lbh alleles are fully functional. LbhloxP mice were then crossed with a Rosa26-Cre line, resulting in ubiquitous deletion of exon 2 and abolishment of LBH protein expression. Mice homozygous for the Lbh null allele (LbhΔ2) displayed normal embryonic development and postnatal growth with morphologies indistinguishable from wild-type littermates. However, mammary gland development, which occurs primarily after birth, was perturbed. Thus, the conditional Lbh allele will be a valuable tool to uncover the currently unknown tissue-specific roles of LBH in postnatal development and disease. genesis 51:491-497.

Original languageEnglish (US)
Pages (from-to)491-497
Number of pages7
JournalGenesis
Volume51
Issue number7
DOIs
StatePublished - Jul 1 2013

Keywords

  • Conditional knockout
  • Cre-loxP
  • LBH
  • Mammary gland development

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

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