Generation of Glucose-Responsive, Insulin-Producing Cells from Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Kamalaveni R. Prabakar, Juan Domínguez-Bendala, R. Damaris Molano, Antonello Pileggi, Susana Villate, Camillo Ricordi, Luca Inverardi

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We sought to assess the potential of human cord blood-derived mesenchymal stem cells (CB-MSCs) to derive insulin-producing, glucose-responsive cells. We show here that differentiation protocols based on stepwise culture conditions initially described for human embryonic stem cells (hESCs) lead to differentiation of cord blood-derived precursors towards a pancreatic endocrine phenotype, as assessed by marker expression and in vitro glucose-regulated insulin secretion. Transplantation of these cells in immune-deficient animals shows human C-peptide production in response to a glucose challenge. These data suggest that human cord blood may be a promising source for regenerative medicine approaches for the treatment of diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)1321-1339
Number of pages19
JournalCell Transplantation
Volume21
Issue number6
DOIs
StatePublished - Sep 3 2012

Keywords

  • Adult stem cells
  • Islets of Langerhans
  • Mesenchymal stem cells (MSCs)
  • Pancreas
  • Umbilical cord blood

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

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