Generation of disease-specific autopsy-confirmed iPSCs lines from postmortem isolated Peripheral Blood Mononuclear Cells

Kinsley Belle, Francelethia S. Shabazz, Karen Nuytemans, David A. Davis, Aleena Ali, Juan Young, William K Scott, Deborah C Mash, Jeffery M Vance, Derek M Dykxhoorn

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Understanding the molecular mechanisms that underlie neurodegenerative disorders has been hampered by a lack of readily available model systems that replicate the complexity of the human disease. Recent advances in stem cell technology have facilitated the derivation of patient-specific stem cells from a variety of differentiated cell types. These induced pluripotent stem cells (iPSCs) are attractive disease models since they can be grown and differentiated to produce large numbers of disease-relevant cell types. However, most iPSC lines are derived in advance of, and without the benefit of, neuropathological confirmation of the donor − the gold standard for many disease classifications and measurement of disease severity. While others have reported the generation of autopsy-confirmed iPSC lines from patient explants, these methods require outgrowth of cadaver tissue, which require additional time and is often only successful ∼50% of the time. Here we report the rapid generation of autopsy-confirmed iPSC lines from peripheral blood mononuclear cells (PBMCs) drawn postmortem. Since this approach doesn't require the propagation of previously frozen cadaver tissue, iPSC can be rapidly and efficiently produced from patients with autopsy-confirmed pathology. These matched iPSC-derived patient-specific neurons and postmortem brain tissue will support studies of specific mechanisms that drive the pathogenesis of neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalNeuroscience Letters
Volume637
DOIs
StatePublished - Jan 10 2017

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Induced Pluripotent Stem Cells
Autopsy
Blood Cells
Cell Line
Cadaver
Neurodegenerative Diseases
Stem Cells
Tissue Donors
Pathology
Technology
Neurons
Brain

Keywords

  • Autopsy
  • Disease modeling
  • iPSCs
  • Neurodegeneration
  • Neuropathological confirmation
  • Parkinson disease
  • PBMCs
  • Postmortem

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Generation of disease-specific autopsy-confirmed iPSCs lines from postmortem isolated Peripheral Blood Mononuclear Cells. / Belle, Kinsley; Shabazz, Francelethia S.; Nuytemans, Karen; Davis, David A.; Ali, Aleena; Young, Juan; Scott, William K; Mash, Deborah C; Vance, Jeffery M; Dykxhoorn, Derek M.

In: Neuroscience Letters, Vol. 637, 10.01.2017, p. 201-206.

Research output: Contribution to journalArticle

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