Generation of an OMgp allelic series in mice

Jae K. Lee, Lauren C. Case, Andrea F. Chan, Yuhong Zhu, Marc Tessier-Lavigne, Binhai Zheng

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The very limited ability to regenerate axons after injury in the mature mammalian central nervous system (CNS) has been partly attributed to the growth restrictive nature of CNS myelin. Oligodendrocyte myelin glycoprotein (OMgp) was identified as a major myelin-derived inhibitor of axon growth. However, its role in axon regeneration in vivo is poorly understood. Here we describe the generation and molecular characterization of an OMgp allelic series. With a single gene targeting event and Cre/FLP mediated recombination, we generated an OMgp null allele with a LacZ reporter, one without a reporter gene, and an OMgp conditional allele. This allelic series will aid in the study of OMgp in adult CNS axon regeneration using mouse models of spinal cord injury. The conditional allele will overcome developmental compensation when employed with an inducible Cre, and allows for the study of temporal and tissue/cell type-specific roles of OMgp in CNS injury-induced axonal plasticity.

Original languageEnglish (US)
Pages (from-to)751-756
Number of pages6
JournalGenesis
Volume47
Issue number11
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Axon regeneration
  • CNS repair
  • Conditional allele
  • Myelin inhibition
  • OMgp

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Generation of an OMgp allelic series in mice'. Together they form a unique fingerprint.

Cite this