General method for the purification of lipids for surface pressure studies. Application to monogalatosyldiglyceride

Pierre Tancrède, Gaétan Chauvette, Roger M. Leblanc

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In order to prepare lipids free of surface-active contaminants ad suitable for the measurement of surface pressure isotherms as general purification method has been developed involving preparative thin-laye chromatography. A detailed description of the procedure and blank experiments carried out with a monolayer trough are presented. The method provides a rapid purification of various classes of lipids, the whole procedure taking less than 1 day. It gives highly reproducible results, better than 2 Å2 per molecule at any surface pressure. As an example of an application, the results obtained with a commercial sample of monogalactosyldiglyceride, a major plant lipid, are reported.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalJournal of Chromatography A
Volume207
Issue number3
DOIs
StatePublished - Mar 27 1981
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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