Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter

Gregory D. Chapman, Chang S. Lim, Roger S. Gammon, Stephen C. Culp, J. Steven Desper, Robert P. Bauman, Judith L. Swain, Richard S. Stack

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Genetic manipulation of the vasculature may offer insights into the pathogenesis of coronary artery disease and may lead to gene therapy for disorders such as restenosis after percutaneous coronary angioplasty. The goal of this study was to develop a percutaneous method for gene transfer into coronary arteries of intact animals. Liposomes were used to facilitate transfection in coronary arteries with a plasmid containing the cDNA encoding luciferase. This reporter was chosen since it is not expressed in mammalian cells, and it can be quantified using a sensitive assay (light production). Mongrel dogs were catheterized, and DNA was delivered to coronary arteries via a porous perfusion balloon system. Luciferase expression was measured 3-5 days after the procedure, when the dogs were killed. Luciferase activity in control arteries (n=12) was no higher than average background activity. Eight of 12 transfected arteries exhibited gene expression, averaging 4.3±2.1 pg luciferase (p<0.01, transfected versus control arteries). In addition, the ability to transfect DNA into femoral arteries without a transfection vehicle was tested. Five dogs were subjected to surgical transfection attempts in their femoral arteries with either DNA alone or DNA plus liposomes. Luciferase was expressed in all 10 femoral arteries; those treated with DNA alone expressed 35.6±8 pg luciferase, and those treated with DNA plus liposomes expressed 42.3±14 pg luciferase (p=0.70). These results demonstrate the use of a percutaneous catheter to achieve gene transfer and expression in coronary arteries of intact dogs and suggest that the efficiency of intra-arterial gene transfer may be similar whether or not a transfection vehicle is used.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalCirculation Research
Volume71
Issue number1
StatePublished - Jul 1 1992
Externally publishedYes

Fingerprint

Luciferases
Coronary Vessels
Catheters
Transfection
DNA
Genes
Femoral Artery
Liposomes
Dogs
Arteries
Gene Expression
Angioplasty
Genetic Therapy
Coronary Artery Disease
Plasmids
Complementary DNA
Perfusion
Light

Keywords

  • Angioplasty
  • Coronary artery
  • Gene transfer
  • Luciferase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Chapman, G. D., Lim, C. S., Gammon, R. S., Culp, S. C., Desper, J. S., Bauman, R. P., ... Stack, R. S. (1992). Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter. Circulation Research, 71(1), 27-33.

Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter. / Chapman, Gregory D.; Lim, Chang S.; Gammon, Roger S.; Culp, Stephen C.; Desper, J. Steven; Bauman, Robert P.; Swain, Judith L.; Stack, Richard S.

In: Circulation Research, Vol. 71, No. 1, 01.07.1992, p. 27-33.

Research output: Contribution to journalArticle

Chapman, GD, Lim, CS, Gammon, RS, Culp, SC, Desper, JS, Bauman, RP, Swain, JL & Stack, RS 1992, 'Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter', Circulation Research, vol. 71, no. 1, pp. 27-33.
Chapman GD, Lim CS, Gammon RS, Culp SC, Desper JS, Bauman RP et al. Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter. Circulation Research. 1992 Jul 1;71(1):27-33.
Chapman, Gregory D. ; Lim, Chang S. ; Gammon, Roger S. ; Culp, Stephen C. ; Desper, J. Steven ; Bauman, Robert P. ; Swain, Judith L. ; Stack, Richard S. / Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter. In: Circulation Research. 1992 ; Vol. 71, No. 1. pp. 27-33.
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