Gene expression profiling reveals alteration of caspase 6 and 14 transcripts in normal skin of keloid-prone patients

Mehdi Nassiri, Heather Woolery-Lloyd, Sharon Ramos, Sharon E. Jacob, Dijana Gugic, Anita Viciana, Paolo Romanelli, George Elgart, Brian Berman, Vladimir Vincek

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Excessive scar formation in keloids points to altered tissue modeling and repair mechanisms. Dysregulation of cytokine and apoptotic cascades and their downstream signaling pathways might have a role in keloid development. Total RNA was isolated from biopsied keloidal tissue and adjacent normal skin of black patients, white patient's scars, and normal skin of black and white patients, with normal wound healing. Apoptosis, cytokine and NFkB pathway microarrays were used to study and compare gene expression levels. Real-time PCR was used to verify microarray results in original samples and a separate, validation-set of samples. Significant differences were observed in the expression levels of members of caspase, cytokines and MAP kinase pathways, between the normal skin of keloid-prone and normal skin of keloid-resistant patients. Specifically, expression of caspase 6, and caspase 14 genes were different between normal skin of keloid-prone individuals and normal skin of keloid-resistant patients. Our results suggest that normal skin of keloid-prone individuals constitutively expresses a distinct gene profile which might contribute to their susceptibility to develop keloids.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalArchives of Dermatological Research
Volume301
Issue number2
DOIs
StatePublished - Feb 1 2009

Keywords

  • Apoptosis
  • Caspase
  • Cytokines
  • Gene expression
  • Keloid
  • Microarray

ASJC Scopus subject areas

  • Dermatology

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